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  4. Human Inhibitor of growth protein 4(ING4) ELISA kit

Human Inhibitor of growth protein 4(ING4) ELISA kit

  • 中文名称：
    人生长抑制蛋白4(ING4)酶联免疫试剂盒
  • 货号：
    CSB-EL011715HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human ING4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ING4 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the ING4 levels in the sample and is measured at 450 nm through a microplate reader.

    ING4 has been identified as a tumor suppressor since it plays a critical role in the regulation of chromatin modification, cell proliferation, angiogenesis, and cell migration. It is frequently down-regulated in various human cancers. Decreased expression or dysregulation of ING4 is widely seen in diverse types of cancers. Ample studies have shown that ING4 is responsible for important cancer hallmarks such as pathologic cell cycle arrest, apoptosis, autophagy, contact inhibition, and hypoxic adaptation, and also affects tumor angiogenesis, invasion, and metastasis.

  • 别名：
    Brain my036 protein ELISA Kit; Candidate tumor suppressor p33 ING 1 homolog ELISA Kit; Candidate tumor suppressor p33 ING1 homolog ELISA Kit; D6Wsu147e ELISA Kit; D6Xrf92 ELISA Kit; ING 1 like protein ELISA Kit; ING 4 ELISA Kit; ING1 like protein ELISA Kit; ING4 ELISA Kit; ING4_HUMAN ELISA Kit; Inhibitor of growth family member 4 ELISA Kit; Inhibitor of growth family member 4 long isoform ELISA Kit; Inhibitor of growth protein 4 ELISA Kit; MGC12557 ELISA Kit; my036 ELISA Kit; p29 ING 4 ELISA Kit; p29 ING4 ELISA Kit; p29ING4 ELISA Kit
  • 缩写：
    ING4
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    47 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ING4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %86-92
    1:2Average %101
    Range %97-103
    1:4Average %96
    Range %92-99
    1:8Average %91
    Range %87-95
  • 回收率：
    The recovery of human ING4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-93
    EDTA plasma (n=4)10499-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.714 2.642 2.678 2.565
    15001.538 1.544 1.541 1.428
    7500.749 0.788 0.769 0.656
    3750.491 0.500 0.496 0.383
    187.50.301 0.312 0.307 0.194
    940.224 0.232 0.228 0.115
    470.183 0.174 0.179 0.066
    00.114 0.112 0.113
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11.
  • 基因功能参考文献：
    1. Splicing type of ING4 affects the translocation of ING4 proteins into the nucleus. PMID: 30403588
    2. Both CELSR2 and ING4 display increased cytoplasmic staining in breast cancer cells compared to benign epithelium, suggesting a possible role of both genes in the pathogenesis of human mammary neoplasia. PMID: 29489009
    3. These results demonstrated that overexpression of ING4 can induce the apoptosis of melanoma cells and CD3+ T cells through signaling pathways such as the Fas/FasL pathway, and that ING4 gene therapy for melanoma treatment is a novel approach. PMID: 29207034
    4. Inhibitor of growth 4 upregulation plus radiotherapy induced synergistic tumor suppression in SPC-A1 xenografts implanted in athymic nude mice. Thus, the restoration of inhibitor of growth 4 function might provide a potential strategy for non-small cell lung cancer radiosensitization. PMID: 27381846
    5. Results found that ING4 expression was significantly reduced in CRC tissues and associated with increased lymph node metastasis, advanced TNM stage and poor overall survival. Also, ING4 suppressed CRC angiogenesis by inhibition of Sp1 expression and transcriptional activity through destabilization and ubiquitin degradation and down-regulation of Sp1 downstream pro-angiogenic factors MMP-2 and COX-2. PMID: 27806345
    6. Low ING4 expression is associated with malignant phenotype and temozolomide chemoresistance in glioblastomas. PMID: 27471108
    7. ING4 directly binds the Miz1 promoter and is required to induce Miz1 mRNA and protein expression during luminal cell differentiation. PMID: 27527891
    8. The oncogenic role of miR-330 in Hepatocellular Carcinoma Cells is linked to downregulation of ING4. PMID: 28050784
    9. ING4 binds double-stranded DNA through its central region with micromolar affinity. PMID: 27926782
    10. results indicate that the combination of ING4 and PTEN may provide an effective therapeutic strategy for HCC PMID: 27421660
    11. ING4 can facilitate cancer cell sensitivity to chemotherapy and radiotherapy. Although ING4 loss is observed for many types of cancers, increasing evidences show that ING4 can be used for gene therapy. In this review, the recent progress of ING4 regulating tumorigenesis is discussed PMID: 26803518
    12. ING4 inhibits CRC invasion and metastasis probably via a switch from mesenchymal marker N-cadherin to epithelial marker E-cadherin through downregulation of Snail1 epithelial-mesenchymal transition (EMT)-inducing transcription factor (EMT-TF). PMID: 26936485
    13. Data show that Ras protein regulates inhibitor of growth protein 4 (ING4)-thymine-DNA glycosylase (TDG)-Fas protein axis to promote apoptosis resistance in pancreatic cancer. PMID: 26544625
    14. MiR-761 directly targeted ING4 and TIMP2. PMID: 26278569
    15. Data suggest a close connection between aberrant ING4 expression and the carcinogenesis of human bladder cells. PMID: 25790869
    16. This review summarizes the recent published literature that investigates the role of ING4 in regulating tumorigenesis and progression, and explores its potential for cancer treatment. [review] PMID: 25968091
    17. SCF(JFK) as a bona fide E3 ligase for ING4 and unraveled the JFK-ING4-NF-kappaB axis as an important player in the development and progression of breast cancer PMID: 25792601
    18. work suggests that ING4 can suppress osteosarcoma progression through signaling pathways such as mitochondria pathway and NF-kappaB signaling pathway and ING4 gene therapy is a promising approach to treating osteosarcoma. PMID: 25490312
    19. The enhanced antitumor activity generated by Ad.RGD-ING4-PTEN was closely associated with activation of the intrinsic and extrinsic apoptotic pathways and additive inhibition of tumor angiogenesis both in vitro and in vivo. PMID: 25571952
    20. The low expression level of ING4 protein was correlated with high-risk gastrointestinal stromal tumors. PMID: 23504291
    21. loss of ING4, either directly or indirectly through loss of Pten, promotes Myc-driven prostate oncogenesis. PMID: 24762396
    22. ING4 level elevation mediated proliferation and invasion inhibition may be tightly associated with the suppression of NF-kappaB signaling pathway. PMID: 24057236
    23. These findings support a critical role for ING4 expression in normal cells in the non-cell-autonomous regulation of tumor growth. PMID: 23604125
    24. ING4 acts as an E3 ubiquitin ligase to induce ubiquitination of p65 and degradation, which is critical to terminate NFkappaB activation. PMID: 23624912
    25. The ING4 Binding with p53 and Induced p53 Acetylation were Attenuated by Human Papillomavirus 16 E6. PMID: 23967213
    26. these findings suggest that ING4 may be a feasible modulator for the MDR phenotype of gastric carcinoma cells PMID: 23969950
    27. KAI1 overexpression increases ING4 expression in melanoma. PMID: 24130172
    28. ING4 may regulate c-MYC translation by its association with AUF1. PMID: 23603392
    29. Report up-regulation of ING4 expression in sarcoid granulomas. PMID: 23181555
    30. ING4 negatively regulates NF-kappaB in breast cancer PMID: 23056468
    31. Data suggested that miR-650 is correlated with the pathogenesis of hepatocellular carcinoma (HCC) and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4. PMID: 22767438
    32. Loss of ING4 expression is associated with lymphatic metastasis in colon cancer. PMID: 23055189
    33. Inhibitor of growth 4 may represent an important biomarker for assessing the severity of breast cancer PMID: 22436625
    34. crystal structure of the ING4 N-terminal domain PMID: 22334692
    35. Data suggest that ING4 may be a promising target for the treatment for ovarian cancer. PMID: 22228137
    36. Mechanism of ING4 mediated inhibition of the proliferation and migration of human glioma cell line U251. PMID: 22078444
    37. In this review, the different properties of ING4 are discussed, and its activities are correlated with different aspects of cell physiology. [Review] PMID: 21971889
    38. Downregulated expression of inhibitor of growth 4 is associated with colorectal cancers. PMID: 21626442
    39. These results sustain the view that ING4 is a tumor suppressor in breast cancer and suggest that ING4 deletion may contribute to the pathogenesis of HER2-positive breast cancer. PMID: 21315418
    40. results suggest that the decreases in nuclear ING4 may play important roles in tumorigenesis, progression and tumor differentiation in head and neck squamous cell carcinoma. PMID: 21310648
    41. EBNA3C negatively regulate p53-mediated functions by interacting with ING4 and ING5. PMID: 21177815
    42. Loss of ING4 is associated with breast carcinoma. PMID: 20707719
    43. Demonstrated decreased ING4 mRNA and expression in 100% (50/50) lung tumour tissues. Furthermore, ING4 expression was lower in grade III than in grades I-II tumours. Reduced ING4 mRNA correlated with lymph node metastasis. PMID: 20716169
    44. Mutations in ING4 is associated with cancer. PMID: 20705953
    45. our data suggest an essential role for ING-4 in human astrocytoma development and progression possibly through regulation of the NF-kappaB-dependent expression of genes involved in tumor invasion PMID: 19775294
    46. A dominant mutant allele of the ING4 tumor suppressor found in human cancer cells exacerbates MYC-initiated mouse mammary tumorigenesis. PMID: 20501848
    47. over-expression of miR-650 in gastric cancer may promote proliferation and growth of cancer cells, at least partially through directly targeting ING4. PMID: 20381459
    48. p29ING4 and p28ING5 may be significant modulators of p53 function. PMID: 12750254
    49. In mice, xenografts of human glioblastoma U87MG, which has decreased expression of ING4, grow significantly faster and have higher vascular volume fractions than control tumours PMID: 15029197
    50. ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells PMID: 15251430

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ING family
  • 数据库链接：

    HGNC: 19423

    OMIM: 608524

    KEGG: hsa:51147

    STRING: 9606.ENSP00000380024

    UniGene: Hs.524210