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  4. Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1 (ADF1)

Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1 (ADF1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1(ADF1),Yeast
  • 货号：
    CSB-YP657196DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1(ADF1),Yeast
  • 货号：
    CSB-EP657196DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1(ADF1),Yeast
  • 货号：
    CSB-EP657196DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1(ADF1),Yeast
  • 货号：
    CSB-BP657196DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Actin-depolymerizing factor 1(ADF1),Yeast
  • 货号：
    CSB-MP657196DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ADF1
  • Uniprot No.：
  • 别名：
    ADF1; At3g46010; F16L2_220Actin-depolymerizing factor 1; ADF-1; AtADF1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-139
  • 氨基酸序列
    MANAASGMAV HDDCKLRFLE LKAKRTHRFI VYKIEEKQKQ VVVEKVGQPI QTYEEFAACL PADECRYAIY DFDFVTAENC QKSKIFFIAW CPDIAKVRSK MIYASSKDRF KRELDGIQVE LQATDPTEMD LDVFRSRAN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin-depolymerizing protein. Stimulates F-actin depolymerization. Involved in plant development, cell organ expansion and flowering by controlling breakdown of thick actin cables. Severs actin filaments or bundles and promotes actin cytoskeleton disassembly. Binds monomeric actin (G-actin) with a marked preference for the ADP-loaded form and inhibits the rate of nucleotide exchange on G-actin.
  • 基因功能参考文献：
    1. Data show that 14-3-3 lambda is required for actin depolymerizing factor 1 (ADF1) phosphorylation and subcellular localization. PMID: 26345162
    2. These results demonstrate a regulatory function of Arabidopsis CDPK6 in the N-terminal phosphorylation of AtADF1. PMID: 23903947
    3. Arabidopsis AtADF1 is functionally affected by mutations on actin binding sites. PMID: 23190411
  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 组织特异性：
    Expressed in vascular tissues of all organs.
  • 数据库链接：

    KEGG: ath:AT3G46010

    STRING: 3702.AT3G46010.2

    UniGene: At.21187