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  4. Recombinant Mouse Cholesterol 24-hydroxylase (Cyp46a1), partial

Recombinant Mouse Cholesterol 24-hydroxylase (Cyp46a1), partial

  • 货号：
    CSB-YP895769MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP895769MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP895769MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP895769MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP895769MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp46a1
  • Uniprot No.：
  • 别名：
    Cyp46a1; Cyp46Cholesterol 24-hydroxylase; CH24H; EC 1.14.14.25; Cholesterol 24-monooxygenase; Cholesterol 24S-hydroxylase; Cytochrome P450 46A1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain. May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole.
  • 基因功能参考文献：
    1. Oral administration of a small dose of the anti-HIV drug efavirenz to a mouse model of Alzheimer's disease ameliorated amyloid pathology in the brain and led to changes supporting therapeutic potential of cholesterol-metabolizing enzyme CYP46A1, its metabolite 24HC and 24HC-mediated liver X receptors transrepression of some of the inflammatory genes in astrocytes and microglia surrounding amyloid plaques. PMID: 28655608
    2. The present study demonstrates the diverse potential effects of CYP46A1 deficiency on brain functions and identifies important proteins that could be affected by this deficiency PMID: 29073233
    3. Increased expression of CYP46A1 in the striatum decreased neuronal atrophy in a Huntington's disease model. PMID: 26912634
    4. under conditions of membrane cholesterol reduction by increased CYP46A1 expression, neurons increase isoprenoid synthesis and sGTPase prenylation. This leads to a reduction in liver X receptor activity PMID: 25084760
    5. EFV enhanced CYP46A1 activity and cerebral cholesterol turnover in animals with no effect on the levels of brain cholesterol. PMID: 24352658
    6. the CYP46 gene locus may predispose to Alzheimer's disease by increasing the 24S-hydroxycholesterol/cholesterol ratio in the brain. PMID: 12232784
    7. constitutes a major tissue-specific pathway for cholesterol turnover in the brain PMID: 12686551
    8. The expression of CYP46A1, as well as other cytochrome P450s involved in cholesterol homeostasis, are potently regulated by histone acetylation status. PMID: 19059217

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Cell junction, synapse, postsynapse. Cell junction, synapse, presynapse. Cell projection, dendrite.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Expressed in high level in the pyramidal cells of the hippocampus, Purkinje cells of the cerebellum, and neuronal cell bodies in layers II/III, V, and VI of the cortex. Expressed in hippocampal and cerebellar interneurons, in retinal ganglion cells, and i
  • 数据库链接：

    KEGG: mmu:13116

    STRING: 10090.ENSMUSP00000021684

    UniGene: Mm.41911