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  4. Recombinant Mouse Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (Pag1), partial

Recombinant Mouse Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (Pag1), partial

  • 中文名称：
    小鼠Pag1重组蛋白
  • 货号：
    CSB-YP664923MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pag1重组蛋白
  • 货号：
    CSB-EP664923MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pag1重组蛋白
  • 货号：
    CSB-EP664923MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pag1重组蛋白
  • 货号：
    CSB-BP664923MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pag1重组蛋白
  • 货号：
    CSB-MP664923MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pag1
  • Uniprot No.：
  • 别名：
    Pag1; Cbp; Pag; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; Transmembrane phosphoprotein Cbp
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • 基因功能参考文献：
    1. our findings illustrate that Cbp is an important regulator of Csk recruitment to the SFK Lyn in Eporesponsive erythroid cells. PMID: 27751872
    2. This study demonstrated that cbp increase in skeletal muscle in muscle atrophy. PMID: 26372908
    3. PAG is constitutively phosphorylated in resting T cells and rapidly dephosphorylated once the TCR is engaged. PMID: 26512138
    4. If Cbp enhances the interaction between c-Src and FAK, Cbp could promote c-Src function when lipid rafts are disrupted. PMID: 24675741
    5. PAG can function as both a positive and a negative regulator of mast cell signaling, depending upon the signaling pathway involved. PAG is a positive regulator of passive systemic anaphylaxis. PMID: 25246632
    6. Data show that the phosphoprotein associated with glycosphigolipid-enriched microdomains (PAG)/Csk binding protein (Cbp) modulates SFK activity in the brain. PMID: 21915273
    7. Cbp plays a role in adult hematopoietic stem cell homeostasis. PMID: 22006020
    8. regulates epithelial-mesenchymal transition in trophoblast stem cells PMID: 21549327
    9. Cbp down-regulation is primarily mediated by epigenetic histone modifications via oncogenic MAPK/PI3K pathways in a subset of cancer cells. PMID: 21388951
    10. expression of Cbp is downregulated during osteoclast differentiation, consequently inhibiting recruitment of Csk to lipid rafts and keeping c-Src activity high in osteoclasts. findings provide a novel paradigm that controls c-Src activity in osteoclasts. PMID: 19874196
    11. CBP plays a role in transiently anchoring Thy-1 to the cytoskeleton. PMID: 19825940
    12. PAG is a bona fide negative regulator of T-cell activation as a result of its capacity to recruit Csk. PMID: 12612075
    13. Csk-binding protein is dispensable for the recruitment of carboxy-terminal Src kinase to the membrane [Csk-binding protein and carboxy-terminal Src kinase ] PMID: 16287865
    14. PTPalpha-/- cells exhibit increased tyrosine phosphorylation of specific proteins, increased Fyn activity, and hyperphosphorylation of Cbp/PAG that promotes its association with C-terminal Src kinase PMID: 16339530
    15. Pronounced, age-dependent reduction in PAG in CD4+ T cells' lipid rafts. PMID: 17559841
    16. These findings indicate a potential role for Cbp as a suppressor of c-Src-mediated tumor progression. PMID: 18498747
    17. PAG regulates PDGFR membrane partitioning and SRC family protein tyrosine kinases mitogenic signaling by modulating GM1 levels within caveolae independently from Csk. PMID: 18695048

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  • 亚细胞定位：
    Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
  • 组织特异性：
    Present in T-cells (at protein level).
  • 数据库链接：

    KEGG: mmu:94212

    STRING: 10090.ENSMUSP00000104021

    UniGene: Mm.23897