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  4. Recombinant Rat Calsequestrin-2 (Casq2)

Recombinant Rat Calsequestrin-2 (Casq2)

  • 货号：
    CSB-YP004557RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004557RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004557RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004557RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004557RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Casq2
  • Uniprot No.：
  • 别名：
    Casq2Calsequestrin-2; Calsequestrin; cardiac muscle isoform
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-413
  • 氨基酸序列
    E EGLNFPTYDG KDRVVSLSEK NLKQVLKRYD LLCLYYHEPV SSDKVAQKQF QLKEIVLELV AQVLEHKNIG FVMVDSRKEA KLAKRLGFSE EGSLYVLKGG RTIEFDGEFA ADVLVEFLLD LIEDPVEIVN NKLEVQAFER IEDQIKLLGF FKNEDSEYYK AFQEAAEHFQ PYIKFFATFD KGVAKKLSLK MNEVGFYEPF MDEPSVIPNK PYTEEELVEF VKEHQRPTLR PLRPEDMFET WEDDLNGIHI VAFAEKSDPD GYEFLEILKQ VARDNTDNPD LSILWIDPDD FPLLVAYWEK TFKIDLFKPQ IGVVNVTDAD SVWMEIPDDD DLPTAEELED WIEDVLSGKI NTEDDDNEDE DDDGDNDNDD DDDDDDNSDE DNDDSDDDDD DDE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
  • 基因功能参考文献：
    1. The exacerbated diastolic Ca(2+) leak and arrhythmogenic activities were related to a diminished expression of calsequestrin along with increased SERCA pump activity, which, in effect, promoted a gain-of-function in RyRs without alterations in SR Ca(2+) load, RyR expression or its Ca(2+) sensitivity. PMID: 28169003
    2. We propose that cocaine accumulates in SR through its affinity for CASQ2 and affects both sasrcoplasmic reticulum Ca(2+) storage and release by altering the normal CASQ2 Ca(2+)-dependent polymerization PMID: 23876860
    3. Data suggest CK2 can phosphorylate CSQ2 co-translationally at biosynthetic sites in rough ER, a process that may result in changes in its subsequent trafficking through the secretory pathway. PMID: 21431367
    4. Mitochondrial uncoupling results in fast transcriptional changes in CASQ2 expression that manifest as compromised Ca(2+) signalling, and these changes can be prevented by ROS scavengers. PMID: 20525644
    5. The role of CASQ2 in calcium (Ca2+) handling in cardiac myocytes can be attributed to its action as a Ca2+ buffer in the lumen of the sarcoplasmic reticulum. PMID: 15486014
    6. Calsequestrin (CASQ2) stabilizes sarcoplasmic reticulum Ca2+ release by inhibiting the RyR2 channel through interaction with triadin (Trd). PMID: 17569730
    7. analysis of how human and rat CASQ2 ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity PMID: 18399795
    8. Egr-1 negatively regulates calsequestrin expression and calcium dynamics in ventricular cells. PMID: 19103607

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  • 亚细胞定位：
    Sarcoplasmic reticulum lumen.
  • 蛋白家族：
    Calsequestrin family
  • 组织特异性：
    Detected in stomach and vas deferens (at protein level).
  • 数据库链接：

    KEGG: rno:29209

    STRING: 10116.ENSRNOP00000021846

    UniGene: Rn.10111