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  4. Chicken Interferon γ,IFN-γ ELISA Kit

Chicken Interferon γ,IFN-γ ELISA Kit

  • 中文名称：
    鸡γ干扰素(IFN-γ)酶联免疫试剂盒
  • 货号：
    CSB-E08550Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        IFNG（Interferon gamma）是一种重要的免疫因子，由多种细胞类型分泌。它可以刺激免疫细胞的活化和增殖，并且可以调节免疫细胞的基因表达和活性。IFNG在维持细胞免疫、抗病毒和抗肿瘤等生物学过程中发挥重要作用。IFNG的含量水平受许多因素影响，如年龄、疾病状态等。
        j9九游会登录入口首页生物所提供的Chicken Interferon γ ,IFN-γ ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的IFNG，其灵敏度为3.125 pg/mL，检测范围为3.125 pg/mL-800 pg/mL。
     
  • 别名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.125 pg/mL-800 pg/mL
  • 灵敏度：
    3.125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-100  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 94  
    Range % 90-98  
  • 回收率：
    The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 93-101  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    800 0.178 0.182 0.180    
    200 0.245 0.233 0.239    
    50 0.457 0.482 0.470    
    12.5 0.848 0.817 0.833    
    3.125 1.396 1.364 1.380    
    0 2.237 2.242 2.240    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

鸡的IFN-gamma试剂盒与其他物种是否有交叉反应性？

A:
（鸡）与人、大鼠和小鼠重组蛋白无交叉反应性。

靶点详情

  • 最新研究进展：
    IFNG，全称为γ-干扰素，是一种由免疫细胞产生的细胞因子，在免疫反应中起重要作用。最新研究发现，IFNG在免疫系统中调节T细胞、B细胞和自然杀伤细胞等免疫细胞的功能。IFNG还在感染和炎症过程中发挥重要作用。研究表明，IFNG水平升高与多种疾病有关，包括自身免疫病、感染性疾病、肿瘤、心血管疾病和神经退行性疾病等。近年来，研究人员正努力探索利用IFNG的治疗作用，例如用IFNG治疗乳腺癌、肺癌和黑色素瘤等。
  • 功能：
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 基因功能参考文献：
    1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
    2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
    3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
    4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
    5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 数据库链接：

    KEGG: gga:396054

    STRING: 9031.ENSGALP00000016086

    UniGene: Gga.916