Chicken adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

文献引用 (3) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Chicken ACTH ELISA Kit was designed for the quantitative measurement of Chicken ACTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.25 pg/mL-50 pg/mL and the sensitivity is 0.125 pg/mL.
缩写：

ACTH
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.25 pg/mL-50 pg/mL
灵敏度：

0.125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	91
	Range %	97-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.