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  4. Chicken secretory immunoglobulin A,SIgA ELISA Kit

Chicken secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名称：
    鸡分泌型免疫球蛋白A(SIgA)酶联免疫试剂盒
  • 货号：
    CSB-E10097Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Chicken SIgA ELISA Kit was designed for the quantitative measurement of Chicken SIgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.234 μg/mL-60 μg/mL and the sensitivity is 0.058 μg/mL.

  • 缩写：
    SIgA
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.234 μg/mL-60 μg/mL
  • 灵敏度：
    0.058 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 86  
    Range % 82-89  
    1:400 Average % 98  
    Range % 92-104  
    1:800 Average % 97  
    Range % 93-101  
    1:1600 Average % 88  
    Range % 84-92  
  • 回收率：
    The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 104 98-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    60 0.345 0.346 0.346    
    15 0.565 0.593 0.579    
    3.75 0.856 0.872 0.864    
    0.94 1.158 1.197 1.178    
    0.234 1.367 1.289 1.328    
    0 1.881 1.825 1.853    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

For the kit CSB-E10097Ch,may I know if it can be tested with tissue samples, specifically small intestine?
Would you have this information available to share?

A:
Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.