Fish trypsin ELISA Kit

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产品详情

产品描述：

This Fish PRSS1 ELISA Kit was designed for the quantitative measurement of Fish PRSS1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.156 ng/mL.
别名：

Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
缩写：

PRSS1
Uniprot No.：

P35031
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	101
	Range %	95-105
1:40	Average %	91
	Range %	84-95
1:80	Average %	106
	Range %	100-111
1:160	Average %	104
	Range %	97-108

回收率：

The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	102	96-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.