Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Human 2,3DPG ELISA Kit was designed for the quantitative measurement of Human 2,3DPG protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 μmol/mL-20 μmol/mL and the sensitivity is 0.078 μmol/mL.
缩写：

2,3DPG
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.312 μmol/mL-20 μmol/mL
灵敏度：

0.078 μmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	92-100
1:2	Average %	98
Range %	92-103
1:4	Average %	95
Range %	90-99
1:8	Average %	90
Range %	85-96

回收率：

The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	84	80-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.