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  4. Human BMP and activin membrane-bound inhibitor homolog(BAMBI) ELISA kit

Human BMP and activin membrane-bound inhibitor homolog(BAMBI) ELISA kit

  • 中文名称：
    人BMP激活素膜结合抑制因子同源物(BAMBI)酶联免疫试剂盒
  • 货号：
    CSB-EL002549HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BAMBI ELISA Kit was designed for the quantitative measurement of Human BAMBI protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    BAMBI ELISA Kit; BAMBI_HUMAN ELISA Kit; BMP and activin membrane bound inhibitor homolog ELISA Kit; BMP and activin membrane-bound inhibitor homolog ELISA Kit; NMA ELISA Kit; Non metastatic gene A protein ELISA Kit; Non-metastatic gene A protein ELISA Kit; Putative transmembrane protein NMA ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BAMBI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 100
    Range % 95-105
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 95
    Range % 90-99
    1:8 Average % 93
    Range % 86-98
  • 回收率：
    The recovery of human BAMBI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-93
    EDTA plasma (n=4) 97 92-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.324 2.495 2.410 2.286
    5 1.551 1.630 1.591 1.467
    2.5 0.950 0.904 0.927 0.803
    1.25 0.572 0.542 0.557 0.433
    0.625 0.363 0.379 0.371 0.247
    0.312 0.264 0.283 0.274 0.150
    0.156 0.224 0.216 0.220 0.096
    0 0.127 0.121 0.124 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Negatively regulates TGF-beta signaling.
  • 基因功能参考文献：
    1. BAMBI promotes macrophage proliferation and differentiation in gliomas. PMID: 29286116
    2. These results showed that BMP2 activated SMAD1/5/8 phosphorylation and up-regulated BAMBI mRNA in human granulosa-lutein cells. PMID: 28578012
    3. Data show that BAMBI is expressed in both circulating CD4+ T cells and plasma and may be characterized as a novel immune regulator for COPD. Strikingly, BAMBI expression was significantly stronger in COPD patients and displayed strong correlations with enhanced plasma TGF-beta 1 levels and the Th17/Treg ratio. PMID: 27549738
    4. Lipopolysaccharides-mediated TLR4 signaling enhances TGF-beta response through downregulating BAMBI during prostatic hyperplasia. PMID: 27243216
    5. Low BAMB1 expression is associated with Non-Small Cell Lung Cancer. PMID: 27197161
    6. TGFbeta target genes including TGFBI, BAMBI, COL3A1 and SERPINE1 are significantly increased in Diamond Blackfan Anemia induced pluripotent stem cells PMID: 26258650
    7. The observations illuminated that BAMBI inhibits adipogenesis by a feedback loop (BAMBI-->beta-catenin nuclear translocation-->BAMBI), which forms with Wnt/beta-catenin signaling. PMID: 24798646
    8. betacatenin expression was downregulated as a result of knocking down of BAMBI, and TGF-beta was downregulated in a similar manner. PMID: 24912656
    9. BAMBI alone or in combination with Smad7 was significantly associated with the OS and DFS. These findings suggest that BAMBI and Smad7 may cooperatively inhibit the TGF-beta signaling, and thus promote the progression of gastric cancer. PMID: 24752577
    10. study demonstrates that LPS- and TNF-alpha-induced NF-kappaBp50-HDAC1 interaction represses BAMBI transcriptional activity, which contributes to TLR4-mediated enhancement of TGF-beta signaling in hepatic stellate cells during liver fibrosis. PMID: 24448807
    11. Overexpression of BAMBI is associated with the growth and invasion of human osteosarcoma. PMID: 23807684
    12. Metformin-mediated Bambi expression in hepatic stellate cells induces prosurvival Wnt/beta-catenin signaling PMID: 22406377
    13. Alterations in BAMBI may play a role in the (patho)physiology of obesity, and manipulation of BAMBI may present a novel therapeutic approach to improve adipose tissue function. PMID: 22187378
    14. Data show that hepatoprotective effects of adiponectin include induction of BAMBI which is reduced in human fatty liver. PMID: 21496456
    15. BAMBI protein is expressed in endothelial cells and is regulated by autophagy and lysosomal degradation. PMID: 20886049
    16. Expression of the TGF pseudoreceptor BAMBI in the human lung, which is upregulated in response to nontypeable Haemophilus influenzae infection in COPD lung tissue in vivo and in vitro. PMID: 20513241
    17. s demonstrate for the first time a nuclear co-translocation of BAMBI with Smad2/3 upon TGF-geta treatment. Moreover, overexpression of BAMBI in an in vitro model led to significantly increased proliferation. PMID: 20189233
    18. beta-catenin interferes with transforming growth factor-beta-mediated growth arrest by inducing the expression of BAMBI PMID: 14660579
    19. Our findings suggest that BAMBI transcription is regulated by TGF-beta signaling through direct binding of SMAD3 and SMAD4 to the BAMBI promoter. PMID: 15240101
    20. BAMBI was linked to a potentially aggressive tumor phenotype and predicted tumor recurrence and cancer-related death in CRC. PMID: 18756595
    21. BAMBI interacts with Wnt receptor Frizzled5, coreceptor LRP6, and Dishevelled2 and increases the interaction between Frizzled5 and Dishevelled2 PMID: 18838381
    22. hypermethylation of the BAMBI gene promoter that leads to BAMBI gene suppression may be one of the epigenetic events affecting the invasiveness or aggressiveness of bladder cancers PMID: 19326429
    23. A colorectal cancer expression profile that includes transforming growth factor beta inhibitor BAMBI predicts metastatic potential. PMID: 19328798
    24. Mechanistically, in addition to interfering with the complex formation between the type I and type II receptors, hBAMBI cooperates with Smad7 to inhibit TGF-beta signaling PMID: 19758997

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    BAMBI family
  • 组织特异性：
    High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.
  • 数据库链接：

    HGNC: 30251

    OMIM: 604444

    KEGG: hsa:25805

    STRING: 9606.ENSP00000364683

    UniGene: Hs.533336