Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Cartilage intermediate layer protein 2(CILP2) ELISA kit

Human Cartilage intermediate layer protein 2(CILP2) ELISA kit

  • 中文名称：
    人软骨间层蛋白2(CILP2)酶联免疫试剂盒
  • 货号：
    CSB-EL005438HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CILP2 ELISA Kit was designed for the quantitative measurement of Human CILP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    CILP2 ELISA Kit; Cartilage intermediate layer protein 2 ELISA Kit; CILP-2) [Cleaved into: Cartilage intermediate layer protein 2 C1; Cartilage intermediate layer protein 2 C2] ELISA Kit
  • 缩写：
    CILP2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CILP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 91-103  
    1:2 Average % 96  
    Range % 90-100  
    1:4 Average % 85  
    Range % 80-90  
    1:8 Average % 92  
    Range % 87-98  
  • 回收率：
    The recovery of human CILP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 84-93  
    EDTA plasma (n=4) 90 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1600 2.153 1.984 2.069 1.986  
    800 1.747 1.712 1.730 1.647  
    400 1.387 1.301 1.344 1.261  
    200 0.965 0.939 0.952 0.869  
    100 0.643 0.604 0.624 0.541  
    50 0.311 0.289 0.300 0.217  
    25 0.192 0.179 0.186 0.103  
    0 0.085 0.080 0.083 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    May play a role in cartilage scaffolding.
  • 基因功能参考文献：
    1. This study aims to analyze the influences of single-nucleotide polymorphism in the NCAN-CILP2 region on non-alcoholic fatty liver disease and plasma lipid levels in the Asian and Pacific ethnic groups. PMID: 26758378
    2. The minor T allele of CILP2 gene and I allele of ACE gene have a protective effect. PMID: 24350279
    3. Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis. PMID: 21880736
    4. Data show no differences in triglyceride or total cholesterol levels in relation to any allelic variants of ANGPTL3, CILP2, or TRIB1 SNPs. PMID: 21691831
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed in articular chondrocytes but not in knee meniscal cartilage cells. Localizes to the intermediate to deep zone of articular cartilage.
  • 数据库链接：

    HGNC: 24213

    OMIM: 612419

    KEGG: hsa:148113

    STRING: 9606.ENSP00000291495

    UniGene: Hs.279574