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  4. Human Interleukin 18,IL-18 ELISA KIT

Human Interleukin 18,IL-18 ELISA KIT

  • 中文名称：
    人白介素18(IL-18)酶联免疫试剂盒
  • 货号：
    CSB-E07450h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL18（白细胞介素18）是一种由免疫细胞产生的细胞因子，在免疫系统中具有重要作用。IL18可促进T细胞的增殖和活化，同时也能促进NK细胞和单核细胞的活化和产生促炎症因子。IL18也参与机体对细菌、病毒和真菌等病原体的免疫应答。IL18在健康人体内的正常含量为0-130pg/mL，但其含量会因各种因素如炎症等而发生变化。
        j9九游会登录入口首页生物所提供的Human Interleukin 18,IL-18 ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、尿液、组织匀浆样本中的IL18，其灵敏度为7.8 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 别名：
    Iboctadekin ELISA Kit; IFN gamma inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; IGIF ELISA Kit; IL 1 gamma ELISA Kit; IL 18 ELISA Kit; IL 1g ELISA Kit; IL-1 gamma ELISA Kit; IL-18 ELISA Kit; IL1 gamma ELISA Kit; IL18 ELISA Kit; IL18 protein ELISA Kit; IL18_HUMAN ELISA Kit; IL1F4 ELISA Kit; IL1g ELISA Kit; IL1gamma ELISA Kit; ILIF4 ELISA Kit; Interferon gamma inducing factor ELISA Kit; Interferon gamma-inducing factor ELISA Kit; Interleukin 1 gamma ELISA Kit; Interleukin 18 (interferon-gamma-inducing factor) ELISA Kit; Interleukin 18 ELISA Kit; Interleukin-1 gamma ELISA Kit; Interleukin-18 ELISA Kit; Interleukin18 ELISA Kit; MGC12320 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 105  
    Range % 99-108  
    1:4 Average % 93  
    Range % 88-98  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率：
    The recovery of human IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-102  
    EDTA plasma (n=4) 92 87-95  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 1.956 1.897 1.927 1.844  
    1000 1.412 1.424 1.418 1.335  
    500 0.876 0.853 0.865 0.782  
    250 0.442 0.465 0.454 0.371  
    125 0.310 0.294 0.302 0.219  
    62.5 0.171 0.179 0.175 0.092  
    31.25 0.119 0.123 0.121 0.038  
    0 0.081 0.085 0.083 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IL-18 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeledIL-18 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
      • Timer
      • Test tubes for dilution.

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    IL18（白细胞介素18）是一种细胞因子，主要通过刺激免疫细胞来调节免疫反应。最近的研究表明，IL18与多种疾病的发生和发展密切相关，如肿瘤、心血管疾病、自身免疫病等。IL18的水平可以用于预测这些疾病的风险，并有望成为治疗这些疾病的新靶点。
  • 功能：
    A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable).
  • 基因功能参考文献：
    1. The results of the linkage disequilibrium test showed that the -137G/C and -607G/T sites had no linkage disequilibrium in the present study. PMID: 29926984
    2. SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755
    3. The adipose tissue IL-18R/IL-18 expression is enhanced in obesity which associates with proinflammatory gene signature and insulin resistance in these individuals. PMID: 28508444
    4. Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to insulin resistance in polycystic ovary syndrome patients. PMID: 29648472
    5. Studied serum level of interleukin 18 (IL18) in HIV and Hepatitis C (HCV) coinfection and monoinfection. Found shown that IL-18 is increased in HIV/HCV coinfection when compared with monoinfection and that this increase is likely due to additive sensing of both viruses. PMID: 27927859
    6. not associated with obesity in this study PMID: 28634744
    7. multiple myeloma patients with IL-18 (rs16944) TT had the highest hemoglobin concentration PMID: 30211233
    8. These results suggest that increased IL-18 secretion from larger numbers of neutrophils elicits mechanical hyperalgesia. PMID: 29353540
    9. The results of this study suggests that IL-18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility. PMID: 29349811
    10. Studied association of single nucleotide polymorphisms of interleukin-18 (IL 18) and lower extremity deep venous thrombosis in a Chinese population. PMID: 29105174
    11. PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606
    12. IL-18 is a susceptibility gene for the disease in southern Chinese children. PMID: 30059753
    13. Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
    14. IL-18 is elevated in polycystic ovary syndrome patients, even in lean ones, and is correlated with insulin resistance and atherosclerotic cardiovascular risk PMID: 29105530
    15. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
    16. The IL18 promoter SNP, rs1946518, is a potential risk factor of periodontitis among young Japanese women. PMID: 29129846
    17. The results of this study suggest that IL18 rs187238 and rs1946518 polymorphisms may be associated with an increased risk of gestational diabetes mellitus as well as with BMI in pregnant women. PMID: 28580570
    18. This is the first study to demonstrate that higher pre-treatment IL-18 is associated with HBeAg seroconversion in patients with HIV/HBV co-infection after 48 weeks of HBV cART, which suggests a role for the inflammasome in a favorable response to treatment. PMID: 28195558
    19. Polymorphisms in the IL-18 gene are associated with viral load in Hepatitis B virus . PMID: 30045250
    20. higher level of IL-18 was observed in the monocyte supernatant of the extremely preterm group during late-onset sepsis PMID: 29042061
    21. Increased production of IL-18 in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590
    22. this review discusses IL-18 biological properties and role in disease pathogenesis PMID: 29247988
    23. IL-18 mRNA expression was increased significantly in chronic myeloid leukemia patients PMID: 29097263
    24. these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals. PMID: 29601578
    25. IL-18 gene variants may serve as important biomarkers in predicting the occurrence of nasopharyngeal carcinoma in Asian population. PMID: 29185028
    26. The association of polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 (IL-18) promoter with risk of hepatocellular carcinoma (HCC). PMID: 28000712
    27. These findings indicate that the IL-18 rs1946518 SNP in the donor liver is a risk factor for developing bacterial infection after liver transplantation. PMID: 29061223
    28. Serum levels of IL-18 in nephropathic patient significantly rise and Serum levels of IL-18 might be a predictor factor of progression of diabetic nephropathy. PMID: 27663212
    29. A higher IL-18 immunostaining in stroma of IBC and LABC was observed in comparison with tumor cells, but stromal immunoexpression was similar between IBC and LABC. PMID: 28766916
    30. IL-18 was increased in lung myeloid cells in severe chronic obstructive pulmonary disease. PMID: 28830544
    31. a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
    32. On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754
    33. Data show that tumor-derived IL-18 induced PD-1 expression on NK cells and is associated with poor prognosis in triple-negative breast cancer. PMID: 28415798
    34. IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248
    35. cardiac IL-18 and circulating IL-18 are involved in the pathogenesis of Fabry cardiomyopathy and left ventricular hypertrophy. PMID: 27888626
    36. Erdr1 is negatively expressed relative to Il-18 in psoriatic lesional skin. PMID: 26990875
    37. rs187238 was not associated with T1D in the Euro-Brazilian population. PMID: 28973736
    38. IL-18 (mRNA) in skeletal muscle appears to be involved in the regulation of intramuscular lipid metabolism and hypertriglyceridemia PMID: 29342149
    39. The evidence for influenza A virus activation via an indirect, IL-18-dependent mechanism indicates that MAIT cells are protective in influenza, and also possibly in any human disease process in which inflammation and IL-18 production occur. PMID: 27543331
    40. The development of urticaria, asthma, dermatitis, rhinitis, and eosinophilic disorders all have demonstrated correlations to increased IL-18 levels either in the tissue or systemically. IL-18 represents a novel site of immune regulation in not only allergic conditions, but also autoimmune diseases and other instances of aberrant immune functioning. PMID: 27496752
    41. Results suggest that a common functional IL18 haplotype associated with heightened proinflammatory responses confers susceptibility to stress-related depression and anxiety through effects on threat-related amygdala function, a risk pathway specific to women. PMID: 27430614
    42. human platelets contain transcripts for the IL-18 gene. They synthesize the cytokine de novo, process and release it upon activation. PMID: 27914933
    43. abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility PMID: 26856767
    44. IL-18 level was found to be significantly elevated in CAD patients compared with control individuals PMID: 28059484
    45. The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
    46. an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
    47. among systemic sclerosis patients in China, dyspnea was significantly associated with IL-18 -607C/A genotype frequency PMID: 27098064
    48. We conclude that IL-18 protein and messenger RNA levels are increased in the endometrium of patients with PCOS, and this effect is correlated with body mass index. PMID: 27313119
    49. Serum IL-18 levels are significantly elevated in depressive patients. PMID: 27455146
    50. IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    HGNC: 5986

    OMIM: 600953

    KEGG: hsa:3606

    STRING: 9606.ENSP00000280357

    UniGene: Hs.83077