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  4. Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

  • 中文名称：
    人前心钠肽(Pro-ANP)酶联免疫试剂盒
  • 货号：
    CSB-E05149h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human Pro-ANP ELISA Kit was designed for the quantitative measurement of Human Pro-ANP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 缩写：
    Pro-ANP
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 86-97
    1:2 Average % 85
    Range % 80-89
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 104
    Range % 96-108
  • 回收率：
    The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-95
    EDTA plasma (n=4) 87 82-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 1.943 1.896 1.920 1.766
    2000 1.452 1.366 1.409 1.255
    1000 0.998 0.968 0.983 0.829
    500 0.646 0.657 0.652 0.498
    250 0.467 0.454 0.461 0.307
    125 0.322 0.306 0.314 0.160
    62.5 0.209 0.203 0.206 0.052
    0 0.155 0.153 0.154 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

为检测Pro-anp水平，所有样本是否同时采集并进行测试？样本可以保存多久？

A:
对于CSB-E05149h（Human Pro Atrial Natriuretic Peptide, Pro-ANP ELISA Kit）-96T，我们建议使用新收集的样本。如果您在这方面有困难，您可以根据实验计划在适当的条件下存储样本。通常情况下，如果您将在5天内使用样本，建议使用2-8摄氏度保存；如果在1个月内使用，建议使用-20摄氏度保存；长期保存建议使用-80摄氏度，但通常不超过2个月。期间避免反复冻融。当然您可以根据自己的实验需求决定是否需要进行分装。对于第二个问题，我们没有进行相关验证，如果条件都允许，建议尽早使用样本。