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  4. Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

  • 中文名称：
    人凝血酶原前体蛋白(PIVKA-II)酶联免疫试剂盒
  • 货号：
    CSB-E13343h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PIVKA-II ELISA Kit was designed for the quantitative measurement of Human PIVKA-II protein in serum, plasma, ascitic fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 缩写：
    PIVKA-II
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, ascitic fluid, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-101  
    1:2 Average % 97  
    Range % 86-101  
    1:4 Average % 96  
    Range % 90-108  
    1:8 Average % 94  
    Range % 82-103  
  • 回收率：
    The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-104  
    EDTA plasma (n=4) 97 91-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.467 2.418 2.443 2.327  
    10 1.962 1.945 1.954 1.838  
    5 1.342 1.311 1.327 1.211  
    2.5 0.823 0.772 0.798 0.682  
    1.25 0.504 0.496 0.500 0.384  
    0.625 0.331 0.321 0.326 0.210  
    0.312 0.201 0.189 0.195 0.079  
    0 0.117 0.114 0.116 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have a question regarding calibration standard in the kit. How this standard protein synthesized or extracted from some source? and how many des-carboxy forms (PIVKAII) it includes?

A:
Thanks for your inquiry.
CSB-E13343h
PIVKA-II, also known as de-gamma-carboxyl prothrombin (DCP), is an abnormal form of prothrombin.
The standard is a special protein. Sorry that the specific preparation method is confidential and we can not disclose it. The standard is provided in the form of lyophilized powder, and the corresponding protective components will be added in it. The concentration is 20 ng/ml after re-suspension.Pls let me know if you have any further questions. Thank you.
Q:

Can you please let me know the below information:
1) Does the kit contain any human derived material? If so, please describe what it is.
2) Does the kit contain any animal derived material? If so please describe what it is. Please let us know if it contains polyclonal or monoclonal antibodies and what species the antibodies are from?

A:
Thanks for your inquiry. CSB-E13343h
Some components in the kit contain BSA.
There are 2 antibodies in the kit. Capture antibody is mouse monoclonal antibody and detection antibody is rabbit polyclonal antibody.Pls let me know if you have any further questions. Thank you.