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Human Spondin-1(SPON1) ELISA kit

  • 中文名称：
    人脊椎蛋白-1(SPON1)酶联免疫试剂盒
  • 货号：
    CSB-EL022599HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human spondin-1 (SPON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SPON1 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SPON1 levels in the sample and is measured at 450 nm through a microplate reader.

    SPON1 is an extracellular matrix protein that is derived from the floor plate of vertebrate embryos and is involved in the growth of axons during embryonic development, differentiation of neural cells, and the promotion of axonal regeneration post-injury. SPON1 could bind the amyloid precursor protein and inhibit its cleavage by β-secretase, leading to cognitive decline in Alzheimer's disease. Aberrant expression of SPON1 has been found in solid tumors. SPON1 has been reported to promote tumor invasion and metastasis in liver cancer.

  • 别名：
    SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
  • 缩写：
    SPON1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-91
    1:2Average %86
    Range %81-92
    1:4Average %92
    Range %87-100
    1:8Average %95
    Range %88-101
  • 回收率：
    The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9386-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.504 2.463 2.484 2.365
    52.006 2.042 2.024 1.905
    2.51.506 1.479 1.493 1.374
    1.250.973 0.897 0.935 0.816
    0.6250.511 0.518 0.515 0.396
    0.3120.285 0.268 0.276 0.157
    0.1560.176 0.187 0.182 0.063
    00.121 0.117 0.119 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
  • 基因功能参考文献：
    1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
    2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
    3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
    4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
    5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
    6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
    7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
    8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
    9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
    10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
    11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
    12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
  • 数据库链接：

    HGNC: 11252

    OMIM: 604989

    KEGG: hsa:10418

    UniGene: Hs.643864