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  4. Human keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Human keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

  • 中文名称：
    人角化细胞内分泌因子(KAF)/双调蛋白(AR)酶联免疫试剂盒
  • 货号：
    CSB-E04496h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human AREG ELISA Kit was designed for the quantitative measurement of Human AREG protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.1 pg/mL.

  • 别名：
    AREG; AREGB; SDGF; Amphiregulin; AR; Colorectum cell-derived growth factor; CRDGF
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.1 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %86-97
    1:2Average %83
    Range %80-86
    1:4Average %91
    Range %85-97
    1:8Average %98
    Range %95-101
  • 回收率：
    The recovery of human KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)104100-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    3002.812 2.836 2.824 2.681
    1501.971 1.965 1.968 1.825
    751.122 1.168 1.145 1.002
    37.50.590 0.571 0.581 0.438
    18.80.385 0.399 0.392 0.249
    9.40.293 0.273 0.283 0.140
    4.70.210 0.216 0.213 0.070
    00.142 0.144 0.143
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
  • 基因功能参考文献：
    1. Amphiregulin contained in non-small-cell lung carcinoma-derived exosomes induces osteoclast differentiation through the activation of EGFR pathway. PMID: 28600504
    2. The studies indicate that HIF2-alpha induces myocardial AREG expression in cardiac myocytes, which increases myocardial ischemia tolerance. PMID: 29483579
    3. AREG mediates hCG-induced StAR expression and progesterone production in human granulosa cells, providing novel evidence for the role of AREG in the regulation of steroidogenesis. PMID: 27113901
    4. Regulatory T-cell-intrinsic amphiregulin is dispensable for suppressive function. PMID: 27040371
    5. No significant correlations emerged for YAP or AREG expression and VIII CN schwannoma volume. PMID: 28430338
    6. Cullin 3 regulates ADAM17-mediated ectodomain shedding of AREG. PMID: 29550478
    7. over-expression of AREG could serve as a novel GC biomarker, and active surveillance of its expression could be a novel approach to GC diagnosis and monitoring. PMID: 27713123
    8. Sprouty2 inhibits amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells. PMID: 27835572
    9. Results show that AREG expression is up-regulated in gastric tumor and its co-expression with TROP2 protein is associated with TNM stage, tumor size, lymph node metastases, and distant metastases. PMID: 28256068
    10. secretion of IL-13 and amphiregulin suggests Intrahepatic Innate lymphoid cells may be recruited to promote resolution and repair and thereby they may contribute to ongoing fibrogenesis in liver disease. PMID: 29261670
    11. EGF-AREG interplay in airway basal cell stem/progenitor cells is one of the mechanisms that mediates the interconnected pathogenesis of all major smoking-induced lesions in the human airway epithelium. PMID: 27709733
    12. AREG expression may be useful for identifying CRTC1-MAML2-positive mucoepidermoid carcinomas and as a marker for favorable prognosis. PMID: 27393417
    13. Amphiregulin enhances VEGF-A production in human chondrosarcoma cells and promotes angiogenesis by inhibiting miR-206 via FAK/c-Src/PKCdelta pathway. PMID: 27826039
    14. Amphiregulin plays an important role in lung neoplasm resistance to amrubicinol PMID: 28476786
    15. EREG and AREG are strongly regulated by methylation, and their expression is associated with CIMP status and primary tumour site. PMID: 27272216
    16. These findings demonstrate the posttranslational regulation of Foxp3 expression by AREG in cancer patients through AREG/EGFR/GSK-3beta signaling, which could lead to Foxp3 protein degradation in Treg cells and a potential therapeutic target for cancer treatment. PMID: 27432879
    17. blocking soluble amphiregulin with a neutralizing antibody also significantly increased apoptotic cell death of HepG2 cells due to treatment with methyl methanesulfonate, cisplatin, or a recombinant p53 adenovirus, suggesting that the function of amphiregulin involved in inhibiting apoptosis may be a common mechanism by which hepatoma cells escape from stimulus-induced apoptosis PMID: 28351301
    18. keratinocyte expression of hAREG elicits inflammatory epidermal hyperplasia PMID: 26519132
    19. Low AREG expression is associated with gastric cancer. PMID: 26884344
    20. RYR2, PTDSS1 and AREG are autism susceptibility genes that are implicated in a Lebanese population-based study of copy number variations in this disease. PMID: 26742492
    21. High Amphiregulin enhances intercellular adhesion molecule-1 expression and promotes tumor metastasis in osteosarcoma. PMID: 26503469
    22. results demonstrate that AREG controls G2/M progression and cytokinesis in keratinocytes via activation of a FoxM1-dependent transcriptional program, suggesting new avenues for treatment of epithelial cancer PMID: 26234682
    23. High expression of amphiregulin is associated with hepatocellular carcinoma. PMID: 26451607
    24. Findings show the involvement of amphiregulin and semaphorin-3A in the improvement of skin innervations and penetration of nerve fibers into the epidermis. PMID: 26201903
    25. Altered AREG expression induced by diverse luteinizing hormone receptor reactivity in granulosa cells may provide a useful marker for oocyte developmental competency PMID: 25911599
    26. Amphiregulin enhances alpha6beta1 integrin expression and cell motility in human chondrosarcoma cells through Ras/Raf/MEK/ERK/AP-1 pathway. PMID: 25825984
    27. Our findings implicate amphiregulin as a critical mediator of the estrogen response in ERalpha-positive breast cancer PMID: 26527289
    28. AR induces hHSC fibrogenic activity via multiple mitogenic signaling pathways, and is upregulated in murine and human NASH, suggesting that AR antagonists may be clinically useful anti-fibrotics in NAFLD. PMID: 25744849
    29. Bradykinin (BK) stimulation of human airway smooth muscle cell increases amphiregulin secretion in a mechanism dependent on BK-induced COX-2 expression. PMID: 26047642
    30. The applied drugs showed remarkable suppression of mTOR expression, which might delay tumor progression. Interestingly, sorafenib and sunitinib increased AREG in HNSCC 11A and 14C PMID: 25862847
    31. Expression profiling demonstrated that AREG-activated EGFR regulates gene expression differently than EGF-activated EGFR PMID: 25454348
    32. This study shows that TGF-alpha uses common and divergent molecular mediators to regulate E-cadherin expression and cell invasion. PMID: 25869072
    33. AREG rs1615111, located in the AREG genomic region, can significantly define different prognostic cohorts in locally advanced GC PMID: 25203737
    34. AREG induces ovarian cancer cell invasion by down-regulating E-cadherin expression PMID: 25261255
    35. During high-pressure ventilation, Nrf2 becomes activated and induces AREG, leading to a positive feedback loop between Nrf2 and AREG, which involves the p38 MAPK and results in the expression of cytoprotective genes. PMID: 24921206
    36. AREG expression was significantly correlated with Edmondson stage and serum AFP level. PMID: 24860833
    37. AREG shedding occurs through a TNF-alpha-converting enzyme-dependent mechanism in diacetyl treated pulmonary epithelial cells. PMID: 24816162
    38. aberrantly activated AREG-EGFR signaling is required for CRTC1-MAML2-positive MEC cell growth and survival, suggesting that EGFR-targeted therapies will benefit patients with advanced, unresectable CRTC1-MAML2-positive MEC. PMID: 23975434
    39. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. PMID: 23851501
    40. IL-1beta-induced amphiregulin release may be involved in the pathogenesis of rheumatoid arthritis. PMID: 24196392
    41. These findings provide mechanistic insight into the regulation of YAP and AREG by RASSF1A in human multistep hepatocarcinogenesis. PMID: 23594797
    42. Data suggest that AREG (amphiregulin), BTC (betacellulin), and EREG (epiregulin) induced prostaglandin E2 production by induction of COX-2 (prostaglandin-endoperoxide synthase 2) through MAP kinase signaling in granulosa cells. PMID: 24092824
    43. Exosome-bound WD repeat protein Monad inhibits breast cancer cell invasion by degrading amphiregulin mRNA. PMID: 23844004
    44. Promoter methylation of AREG is associated with glioblastoma. PMID: 23624749
    45. AREG plays pro-neoplastic roles in colorectal carcinogenesis PMID: 23263765
    46. EREG-AREG and NRG1, which are members of the epidermal growth factor (EGF) family, seem to modulate Behcet's disease susceptibility through main effects and gene-gene interactions PMID: 23625463
    47. we did not find a correlation between the presence of a K-ras mutation and the presence of Epiregulin and Amphiregulin in colon cancer tissue. PMID: 23885463
    48. Regulation of amphiregulin gene expression by beta-catenin signaling in human hepatocellular carcinoma cells. PMID: 23285165
    49. Human antigen R-mediated mRNA stabilization is required for ultraviolet B-induced autoinduction of amphiregulin in keratinocytes. PMID: 23430747
    50. Polycystin-1 regulates amphiregulin expression through CREB and AP1 signalling, which has implications in ADPKD cell proliferation PMID: 22570239

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  • 亚细胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Amphiregulin family
  • 数据库链接：

    HGNC: 651

    OMIM: 104640

    KEGG: hsa:374

    STRING: 9606.ENSP00000370227

    UniGene: Hs.270833