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  4. Human lipolysaccharide binding protein,LBP ELISA Kit

Human lipolysaccharide binding protein,LBP ELISA Kit

  • 中文名称：
    人脂多糖结合蛋白(LBP)酶联免疫试剂盒
  • 货号：
    CSB-E09629h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO’s Human lipopolysaccharide-binding protein (LBP) ELISA Kit is suitable for the quantitative detection of LBP content in human serum, plasma, or tissue homogenates. LBP is a carrier protein of the endotoxins' recognition by the host. It is produced by hepatocytes and released into the bloodstream as a consequence of Gram-negative infection. LBP conveys LPS to the cellular surface, forming a ternary complex with a cluster of CD14. CD14 facilitates the transfer of LPS to the TLR4/MD2 complex, activating LPS-TLR4 signaling that elicits proinflammatory responses. It is significantly up-regulated during acute-phase reaction following the initial inflammatory responses, promoting, along with (bactericidal/permeability-increasing protein), non-inflammatory clearance of LPS, and ultimate resolution of LPS-induced inflammation. Additionally, LBP mediates endothelial dysfunction and is required for apoptosis in tubular cells.

    The detection mechanism of this kit is based on the sandwich ELISA technique and enzyme-substrate chromogenic reaction. The formed pre-coated LBP antibody/LBP/Biotin-conjugated LBP antibody complex is labeled by HRP-avidin and then develops a color reaction after the addition of The TMB substrate solution. The color intensity can be measured at 450 nm via a microplate reader.

  • 别名：
    BPI fold containing family D, member 2 ELISA Kit; Bpifd2 ELISA Kit; LBP ELISA Kit; LBP_HUMAN ELISA Kit; LBP1 ELISA Kit; Lipopolysaccharide binding protein ELISA Kit; Lipopolysaccharide-binding protein ELISA Kit; LPS binding protein ELISA Kit; Ly88 ELISA Kit; MGC22233 ELISA Kit; OTTHUMP00000030965 ELISA Kit; RP23-407H16.4 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 μg/mL-40 μg/mL
  • 灵敏度：
    0.156 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-102
    1:2Average %95
    Range %91-99
    1:4Average %99
    Range %96-108
    1:8Average %94
    Range %89-98
  • 回收率：
    The recovery of human LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9992-108
    EDTA plasma (n=4)8480-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2AverageCorrected
    402.795 2.694 2.745 2.613
    202.232 2.131 2.182 2.050
    101.464 1.443 1.454 1.322
    50.812 0.823 0.818 0.686
    2.50.425 0.415 0.420 0.288
    1.250.300 0.311 0.306 0.174
    0.6250.222 0.218 0.220 0.088
    00.132 0.131 0.132 　
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LBP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled LBP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
  • 基因功能参考文献：
    1. This study shows a remarkable reversal of amyloid fibrin formation by LBP addition to the plasma of Parkinson's Disease patients . PMID: 29494603
    2. Serum LBP levels are associated with arterial stiffness, independent of obesity and traditional cardiovascular risk factors, especially in men with type 2 diabetes. PMID: 28486964
    3. novel observation that sCD14 compared with lipopolysaccharide binding protein, offers a preferred target to ameliorate TLR especially TLR4-induced inflammation and insulin resistance in human obesity and metabolic syndrome PMID: 26880233
    4. LBP, an endotoxemia associated protein might be used as an inflammatory biomarker of both infectious and non-infectious origins in HCV-infected subjects PMID: 28107471
    5. Data show that after matching for gender, age, and body mass index (BMI), serum lipopolysaccharide-binding protein (LBP) does not improve prediction of the development of type 2 diabetes mellitus (T2DM) independently. PMID: 25753130
    6. The main findings of this study are that, in acute stroke patients, levels of LBP, IL-10, IL-6 and CRP show a different time course in patients with and without post-stroke infection. PMID: 25613713
    7. Serum LBP level is significantly elevated in polycystic ovary syndrome women and is associated with insulin resistance. PMID: 26799617
    8. LBP serves not only as an extracellular LPS shuttle but in addition facilitates intracellular transport of LPS. PMID: 26804480
    9. LBP level was not significantly different in neutropenic systemic inflammatory response syndrome patients and sepsis patients. PMID: 26046926
    10. Report increased secretion of Fetuin A, LBP and HMGB-1 from subcutaneous adipose tissue in metabolic syndrome. PMID: 25978344
    11. Low levels of microbial translocation marker LBP are associated with sustained viral response after anti-HCV treatment in HIV-1/HCV co-infected patients PMID: 25785448
    12. serum level elevated in late-onset neonatal sepsis in very low birth weight infants PMID: 25014514
    13. Adjusting for body mass index and waist circumference, LBP levels remained significantly increased in metabolic syndrome and increased with increasing numbers of MetS risk factors. PMID: 25063948
    14. study suggested that elevated plasma LBP was associated with an increased risk of developing MetS among middle-aged and older Chinese, especially in normal-weight individuals. PMID: 24906952
    15. In preterm neonates born to asymptomatic women with PPROM, LBP in cord blood at delivery is an excellent diagnostic biomarker of Fetal inflammatory response syndrome/funisitis with prognostic potential. PMID: 24335151
    16. report pre-ART IL-6 and LBP levels as well as IL-6, LBP and I-FABP levels during IRIS-event as potential biomarkers in TB-IRIS PMID: 24312369
    17. In childnre, LBP was independently associated with BMI and with measures of obstructive sleep apnea severity as well as with metabolic dysfunction, particularly insulin resistance as indicated by the homeostasis model assessment of insulin resistance. PMID: 24276451
    18. Suggest that the serum LBP concentration constitutes a reliable biologic marker for the diagnosis of a febrile urinary tract infection in children. PMID: 23463341
    19. Mutations in the lipopolysaccharide binding protein impair innate immunity. PMID: 24120359
    20. Serum LBP concentrations increased with age. Overweight, obesity, and having metabolic syndrome (particularly, low HDL cholesterol levels) were associated with higher LBP concentrations PMID: 23349936
    21. expression in oral keratinocytes is stimulated by Porphyromonas gingivalis LPS PMID: 22736337
    22. Human lipopolysaccharide-binding protein (LBP) and CD14 independently deliver triacylated lipoproteins to Toll-like receptor 1 (TLR1) and TLR2 and enhance formation of the ternary signaling complex PMID: 23430250
    23. LBP is a surrogate marker of microbial translocation in association with physical functioning of older adults. PMID: 22960476
    24. Association of lipopolysaccharide-binding protein gene polymorphisms with cerebral infarction in a Chinese population PMID: 22476641
    25. our study demonstrated that an elevated LBP level of >15000 ng/ml may serve as a biomarker for the prediction and monitoring of aGVHD. PMID: 22552880
    26. During the first 14 days of postoperative sepsis, LBP plasma concentrations showed a time course that was very similar to CRP with a high concordance in the pattern of day-to-day changes PMID: 21901123
    27. The availability of commercial methods for the automated measurement of the soluble CD14 subtype presepsin and lipopolysaccharide binding protein represent a challenge for the evaluation in clinical practice of reliable markers of neonatal sepsis. PMID: 21740312
    28. Aseptic trauma primes the innate immune system for the posttraumatic release of lipopolysaccharide binding protein and sCD14 PMID: 21722015
    29. Cytokine concentrations in amniotic fluid during the mid-trimester did not differ with parity or fetal gender. IL-6, IL-8, and LBP levels appeared stable with gestational week (GW), whereas GW significantly influenced TNF-alpha concentrations. PMID: 21702700
    30. LBP measurements performed shortly after preterm premature rupture of membranes, are not of value either in the prediction of newborn's infection, or in the prognosis of latency period duration. PMID: 21353369
    31. This study highlights the LBP rs2232596 and CD14 rs4914 polymorphisms as biomarkers for elevated colorectal carcinoma susceptibility in the Chinese Han population PMID: 21633598
    32. High LBP is associated with Crohn's disease. PMID: 20865702
    33. Elevated circulating LBP was associated with obesity, metabolic syndrome, and type 2 diabetes in apparently healthy Chinese. PMID: 20530747
    34. The association of the polymorphisms c.291C>T and c.613A>G suggest a role of LBP in the disease manifestation of infective endocarditis. PMID: 19560454
    35. Serial LBP serum measurements may offer a clinically useful biomarker for identification of patients with severe sepsis having the worst outcomes and the highest probability of developing sepsis-induced ARDS PMID: 19718443
    36. carboxyl-terminal domain of these closely related endotoxin-binding proteins dictates the route and host responses to complexes they form with endotoxin. PMID: 11773072
    37. a new and reliable infection marker after kidney transplantation PMID: 11976738
    38. Identification of single amino acid residues essential for the binding of lipopolysaccharide (LPS) to LPS binding protein (LBP) residues 86-99 by using an Ala-scanning library PMID: 11991204
    39. plasma factor LBP and cell surface receptor CD14 were necessary for LPS activation of p38, which was tightly associated with LPS priming of the PMN respiratory burst PMID: 12117913
    40. Besides a role in the detoxification of bacterial toxin present in the circulation, LBP-chylomicron complexes may be part of a local defense mechanism of the intestine against translocated bacterial toxin. PMID: 12538700
    41. innate immune recognition of LTA via LBP, CD14, and TLR-2 represents an important mechanism in the pathogenesis of systemic complications in the course of infectious diseases brought about by Gram-positive pathogens. while TLR-4 and MD-2 are not involved. PMID: 12594207
    42. Single nucleotide polymorphism of the LBP gene is not assciated with complicated sepsis after trauma. PMID: 12615620
    43. data support the hypothesis that lipopolysaccharide binding protein can inhibit cell responses to lipopolysaccharide(LPS) by inhibiting LPS transfer from membrane CD14 to the Toll-like receptor 4-MD-2 signaling receptor PMID: 12754215
    44. plays an essential role in the innate immune response to Gram-positive pneumococci PMID: 12932360
    45. In critically ill neonates aged over 48 h and children lipopolysaccharide binding protein is a better marker than procalcitonin, interleukin-6 and C-reactive protein. PMID: 15127192
    46. Dual role of LBP and CD14 in initiation of proinflammatory signaling and clearance or neutralization of LPS. PMID: 15618154
    47. studies suggest that SP-A could contribute to modulate Re-LPS responses by altering the competence of the LBP-CD14 receptor complex PMID: 15932345
    48. LBP mediates the fusion of lipid membranes and LPS aggregates. PMID: 16303759
    49. Human pulmonary LBP acts as an important modulator of the LPS response in the respiratory tract in vivo. PMID: 16493079
    50. Significantly elevated serum concentrations of LBP and sCD14 are found in severe sepsis patients. PMID: 16512634

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule membrane.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 组织特异性：
    Detected in blood serum (at protein level).
  • 数据库链接：

    HGNC: 6517

    OMIM: 151990

    KEGG: hsa:3929

    STRING: 9606.ENSP00000217407

    UniGene: Hs.154078