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  4. Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

Human macrophage inflammatory protein 5,MIP-5 ELISA Kit

  • 中文名称：
    人巨噬细胞炎性蛋白5(MIP-5)酶联免疫试剂盒
  • 货号：
    CSB-E07997h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human CCL15 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL15 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL15 levels in the sample and is measured at 450 nm through a microplate reader.

    CCL15 is a potent chemoattractant for leukocytes and endothelial cells (ECs). CCL15 exerts its role through binding to CCR1 and CCR3. It induces chemotactic migration of monocytes, dendritic cells, T lymphocytes, and eosinophils and is primarily expressed in the colon and liver. CCL15 also induces the expression of proinflammatory cytokines and tissue factors in activated monocytes. A study has demonstrated that CCL15 induces expression of ICAM-1 via activation of the JAK2/STAT3 signal pathway. CCL15 plays a role in the pathogenesis of cancer and other diseases, such as colorectal cancer, hepatocellular carcinoma, atherosclerosis, asthma, and rheumatoid arthritis.

  • 别名：
    C-C motif chemokine 15 ELISA Kit; C-C motif chemokine ligand 15 ELISA Kit; CC chemokine 3 ELISA Kit; CCL15 ELISA Kit; CCL15(29-92) ELISA Kit; CCL15_HUMAN ELISA Kit; Chemokine (C C motif) ligand 15 ELISA Kit; Chemokine CC-2 ELISA Kit; Chemokine CC2 ELISA Kit; HCC-2 ELISA Kit; HCC2 ELISA Kit; HMRP 2B ELISA Kit; HMRP2B ELISA Kit; Leukotactin 1 ELISA Kit; Leukotactin-1 ELISA Kit; LKN-1 ELISA Kit; LKN1 ELISA Kit; Macrophage inflammatory protein 5 ELISA Kit; MIP 1D ELISA Kit; MIP-1 delta ELISA Kit; MIP-1D ELISA Kit; MIP-5 ELISA Kit; MIP1 delta ELISA Kit; MIP1d ELISA Kit; MIP5 ELISA Kit; MRP 2B ELISA Kit; Mrp-2b ELISA Kit; Mrp2b ELISA Kit; NCC 3 ELISA Kit; NCC-3 ELISA Kit; NCC3 ELISA Kit; New CC chemokine 3 ELISA Kit; OTTHUMP00000163955 ELISA Kit; SCYA15 ELISA Kit; SCYL3 ELISA Kit; Small inducible cytokine A15 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 15 ELISA Kit; Small-inducible cytokine A15 ELISA Kit; SY15 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
  • 基因功能参考文献：
    1. CCL15 secreted from SMAD4-deficient xenografted human colorectal cancer cells recruited CCR1(+) cells, promoting their metastatic activities to the lung. Lung metastases from colorectal cancer patients revealed that CCL15 expression correlated with loss of SMAD4. CCL15-positive metastases recruited approximately 1.9 times more numbers of CCR1(+) cells than CCL15-negative metastases. PMID: 27492974
    2. CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis. PMID: 26501423
    3. Orthotopic animal models of HCC were established to investigate the role of CCL15 in hMSCs migration toward HCC in vivo. Both histological and flow cytometric analysis showed that significantly fewer hMSCs localized within 97H-CCL15-shRNA xenografts compared with 97H-green fluorescent protein xenografts after intravenous delivery PMID: 26763650
    4. Loss of SMAD4 was significantly associated with CCL15 expression. PMID: 26341919
    5. follicular thyroid carcinoma (FTC) might induce tumor associated macrophages (TAMs) infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from follicular adenoma PMID: 26875556
    6. two inflammatory cytokines, MIP-1delta and MIP-3alpha, are able to increase mesenchymal stromal cells migration in vitro PMID: 25579056
    7. we investigated a role of CCL15 in upregulating ICAM-1 in endothelial cells PMID: 23690481
    8. CCL15 is elevated in human asthma and correlates with disease severity . PMID: 22092970
    9. Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551
    10. increased secretion by macrophages during Mycobacterium tuberculosis infection PMID: 20016943
    11. truncation of NH2-terminal amino acid residues increases agonistic potency on CC chemokine receptors 1 and 3 PMID: 11832479
    12. Lkn-1 activates the ERK pathway by transducing the signal through G(i)/G(o) protein, PLC, PKC delta and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes PMID: 12759139
    13. These observations demonstrate that the two NF-kappaB binding sites are essential for phorbol myristate acetate-induced leukotactin-1 (Lkn-1)/CCL15 expression in human monocytes. PMID: 15179048
    14. CCL15(25-92) has in vitro and in vivo angiogenic activity PMID: 15251437
    15. alanine-aspartic acid residues preceding the first cysteine at the NH(2)-terminus are essential for the binding and biological activity of leukotactin-1 PMID: 15894113
    16. Compared with full-length CCL15, proteolytically processed CCL15 isoforms with N-terminal deletions display increased potency to induce calcium fluxes and chemotactic activity on monocytes and to induce adhesiveness of mononuclear cells to fibronectin. PMID: 16034099
    17. Transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells. PMID: 16364464
    18. Results point to an involvement of the CCL15-CCR1 axis in the pathophysiology of chronic renal failure. PMID: 16737685
    19. factor NF-kappaB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1 PMID: 17296613

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
  • 数据库链接：

    HGNC: 10613

    OMIM: 601393

    KEGG: hsa:6359

    STRING: 9606.ENSP00000432034

    UniGene: Hs.272493