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  4. Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

  • 中文名称：
    人Ⅰ型前胶原N端前肽(PⅠNP)酶联免疫试剂盒
  • 货号：
    CSB-E11226h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PⅠNP ELISA Kit was designed for the quantitative measurement of Human PⅠNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL .

  • 别名：
    procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
  • 缩写：
    PⅠNP
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.68 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 97  
    Range % 89-102  
    1:200 Average % 90  
    Range % 83-97  
    1:400 Average % 99  
    Range % 94-105  
    1:800 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of human PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-99  
    EDTA plasma (n=4) 101 95-107  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1200 2.191 2.287 2.239 2.086  
    600 1.885 1.896 1.891 1.738  
    300 1.527 1.507 1.517 1.364  
    150 1.001 1.042 1.022 0.869  
    75 0.692 0.648 0.670 0.517  
    37.5 0.499 0.472 0.486 0.333  
    18.75 0.272 0.288 0.280 0.127  
    0 0.152 0.154 0.153 　  
  • 数据处理：
  • 货期：
    3-5 working days

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