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  4. Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

  • 中文名称：
    小鼠丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)酶联免疫试剂盒
  • 货号：
    CSB-E16539m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ALT/GPT ELISA Kit was designed for the quantitative measurement of Mouse ALT/GPT protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.

  • 别名：
    Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
  • 缩写：
    ALT/GPT
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, cell lysates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %83-95
    1:2Average %97
    Range %92-101
    1:4Average %106
    Range %98-113
    1:8Average %94
    Range %87-97
  • 回收率：
    The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)10295-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    102.645 2.566 2.606 2.421
    52.015 1.855 1.935 1.750
    2.51.194 1.155 1.175 0.990
    1.250.777 0.796 0.787 0.602
    0.6250.492 0.461 0.477 0.292
    0.3120.335 0.344 0.340 0.155
    0.1560.278 0.288 0.283 0.098
    00.191 0.178 0.185 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • 基因功能参考文献：
    1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
    2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
    3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
  • 组织特异性：
    Mainly expressed in liver, intestine, colon and white adipose tissue.
  • 数据库链接：