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  4. Mouse Alpha-fetoprotein,AFP ELISA Kit

Mouse Alpha-fetoprotein,AFP ELISA Kit

  • 中文名称：
    小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)酶联免疫试剂盒
  • 货号：
    CSB-E08282m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse Alpha-fetoprotein (AFP) ELISA Kit is intended to measure mouse AFP in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify mouse AFP concentration. The intensity of the colored product is directly proportional to the concentration of AFP present in the sample. This mouse AFP ELISA kit has high specificity and excellent sensitivity.

    AFP is the most abundant serum protein before birth, and its levels fall to very low but detectable levels after birth. Healthy adults should have very low AFP levels. It is produced by the fetal yolk sac, fetal and regenerating adult liver, and fetal gastrointestinal epithelium. AFP is an oncofetal glycoprotein expressed in the yolk sac tumor and its variants, including hepatoid and endometrioid yolk sac tumors. Elevated serum AFP concentrations have been used as a clinical biomarker for the detection of liver cancer or ovarian or testicular cancers, as well as liver diseases such as cirrhosis and hepatitis.

  • 别名：
    AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    1.23 ng/ml-900 ng/ml.
  • 灵敏度：
    1.23 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 线性度：

      

  • 回收率：

      

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds estrogens, fatty acids and metals.
  • 基因功能参考文献：
    1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
    2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
    3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
    4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
    5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
    6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
    7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
    8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
    9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
    10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
    11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
    12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
    13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
    14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
    15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
    16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma.
  • 数据库链接：