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  4. Mouse Aspartate Aminotransferase (AST) ELISA kit

Mouse Aspartate Aminotransferase (AST) ELISA kit

  • 中文名称：
    小鼠天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒
  • 货号：
    CSB-E12649m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse GOT2 ELISA Kit was designed for the quantitative measurement of Mouse GOT2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.

  • 别名：
    Got2 ELISA kit; Got-2Aspartate aminotransferase ELISA kit; mitochondrial ELISA kit; mAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.7 ELISA kit; Fatty acid-binding protein ELISA kit; FABP-1 ELISA kit; Glutamate oxaloacetate transaminase 2 ELISA kit; Kynurenine aminotransferase 4 ELISA kit; Kynurenine aminotransferase IV ELISA kit; Kynurenine--oxoglutarate transaminase 4 ELISA kit; Kynurenine--oxoglutarate transaminase IV ELISA kit; Plasma membrane-associated fatty acid-binding protein ELISA kit; FABPpm ELISA kit; Transaminase A ELISA kit
  • 缩写：
    GOT2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    1.56 mIU/mL-100 mIU/mL
  • 灵敏度：
    0.39 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %93
    Range %87-98
    1:4Average %91
    Range %89-94
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of mouse AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)9894-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    1002.915 2.898 2.907 2.720
    502.475 2.432 2.454 2.267
    252.015 2.002 2.009 1.822
    12.51.376 1.342 1.359 1.172
    6.250.731 0.717 0.724 0.537
    3.120.472 0.445 0.459 0.272
    1.560.312 0.301 0.307 0.120
    00.189 0.185 0.187 　
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse AST antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled AST antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
  • 基因功能参考文献：
    1. GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in EA PMID: 29107957
    2. it is quite likely that the down regulation of GOT2 after blast exposure is playing a significant role in mitochondrial dysfunction after blast exposure PMID: 23600763
    3. The role of mAspAT in vivo is to control the levels of endogenous kynurenic acid (KYNA) through modulation of the mAspAt in the mouse brain. PMID: 20977429
    4. KAT IV inhibited by equimolar tryptophan and aspartate, and half-life is 6.5 minutes at 65 degree C. PMID: 20482848
  • 亚细胞定位：
    Mitochondrion matrix. Cell membrane.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 组织特异性：
    Detected in brain (at protein level).
  • 数据库链接：