Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse C-Peptide ELISA Kit

Mouse C-Peptide ELISA Kit

  • 中文名称：
    小鼠C肽(C-Peptide)酶联免疫试剂盒
  • 货号：
    CSB-E05068m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse C-Peptide ELISA Kit was designed for the quantitative measurement of Mouse C-Peptide protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.86 ng/mL-50 ng/mL and the sensitivity is 1.786 ng/mL.

  • 缩写：
    C-Peptide
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.125 ng/ml-50 ng/ml
  • 灵敏度：
    0.625 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 85-99  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 95  
    Range % 87-106  
    1:8 Average % 95  
    Range % 89-100  
  • 回收率：
    The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 95 91-103  
                 
                 
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse C-peptide antibody.
      • Six vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial HRP-conjugated C-peptide antibody(6 ml/bottle) ---Bind to the C-peptide in the samples or standards and react with the substrate to make the solution chromogenic.
      • One vial Wash Buffer (20x concentrate) (15ml/bottle) ---Wash away unbound or free substances.
      • Substrate A (1 x 7 ml)
      • Substrate B (1 x 7 ml)
      • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
      • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    显示更多

    收起更多

  • 数据处理：
  • 货期：
    3-5 working days

产品评价