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  4. Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名称：
    小鼠肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒
  • 货号：
    CSB-E14404m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CK-MB ELISA Kit was designed for the quantitative measurement of Mouse CK-MB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 mU/mL-80 mU/mL and the sensitivity is 0.312 mU/mL.

  • 缩写：
    CK-MB
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.25 mU/mL-80 mU/mL
  • 灵敏度：
    0.312 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 80-99  
    1:2 Average % 95  
    Range % 86-105  
    1:4 Average % 91  
    Range % 82-100  
    1:8 Average % 93  
    Range % 82-101  
  • 回收率：
    The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-105  
    EDTA plasma (n=4) 95 89-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    80 2.912 2.787 2.850 2.684  
    40 2.386 2.374 2.380 2.214  
    20 1.617 1.664 1.641 1.475  
    10 0.846 0.895 0.871 0.705  
    5 0.553 0.523 0.538 0.372  
    2.5 0.318 0.336 0.327 0.161  
    1.25 0.246 0.252 0.249 0.083  
    0 0.164 0.168 0.166 　  
  • 数据处理：
  • 货期：
    3-5 working days

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