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  4. Mouse Interleukin 1α,IL-1α ELISA kit

Mouse Interleukin 1α,IL-1α ELISA kit

  • 中文名称：
    小鼠白介素1α(IL-1α)酶联免疫试剂盒
  • 货号：
    CSB-E04621m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL1A (白细胞介素1α，又称为淋巴母细胞刺激因子) 是一种由多种细胞分泌的蛋白质。它是一种激素，参与体内炎症反应的调节，并且也参与到骨髓细胞分化和骨吸收。IL1A 与其他蛋白质互相作用，参与调控免疫反应、发炎反应和细胞增殖等过程。它的过度表达与多种疾病相关，包括炎症性疾病、自身免疫疾病、某些癌症和心血管疾病等。
        j9九游会登录入口首页生物所提供的Mouse Interleukin 1α,IL-1α ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的IL1A，其灵敏度为0.58 pg/ml，检测范围为2.35 pg/ml - 150 pg/ml。
     
  • 别名：
    Il1aInterleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.35 pg/ml - 150 pg/ml
  • 灵敏度：
    0.58 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

      

  • 线性度：

     
     
      

  • 回收率：

      

  • 标准曲线：

       

  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    IL1A是一种细胞因子，具有调节炎症和免疫反应的作用。最新研究发现，IL1A不仅在炎症和免疫相关疾病中发挥重要作用，还可能参与癌症的发生和进展。
  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能参考文献：
    1. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
    2. Together, these data suggest that caspase-11/IL-1alpha pathway plays an important role in defending against Klebsiella pneumoniae by recruiting neutrophils in the early stage of infection. PMID: 28939441
    3. These data highlight an important interdependency between the potent pro-inflammatory cytokine IL1A and Fshr expression. PMID: 28337831
    4. Since neither IL-1alpha nor IL-1beta depletions completely rescued the phenotype, we believe that IL-1alpha and IL-1beta have a similar and probably complementary role in FHF progression PMID: 28953903
    5. These results suggested that Streptococcus pneuomoniae PLY induces the influx of calcium in Streptococcus pneumoniae-infected macrophages, followed by calpain activation and subsequent IL-1alpha maturation and secretion. PMID: 28630064
    6. In response to chemically induced colitis, this microbial landscape promoted the release of IL-1alpha, which acted as a critical driver of colitis and colitis-associated cancer. PMID: 27775548
    7. our results suggest that mature IL-1alpha induced by hS100A7 is via RAGE-p38 MAPK and calpain-1 pathway in keratinocyte and this mechanism may play an important role during psoriasis. PMID: 28060905
    8. Il-1 signaling pathway has a key role in abdominal aortic aneurysm formation in mouse model of Kawasaki disease. PMID: 26941015
    9. endothelial cells were identified as the primary cellular source of G-CSF during OPC, which responded to IL-1alpha that was released from keratinocytes in the infected tissue. PMID: 27632536
    10. Key aspects of IL-1alpha biology and regulation especially with regard to inflammation are reviewed. Review. PMID: 27434011
    11. data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1 PMID: 27226621
    12. IL-1alpha signaling and DNA damage is important for triggering a sterile inflammatory cascade . PMID: 26439902
    13. As a dual function cytokine, IL-1alpha may contribute to the induction of CHOP intracellularly, while IL-1alpha released from necrotic cells accelerates steatohepatitis via induction of inflammatory cytokines by neighboring cells. PMID: 26022690
    14. These data demonstrate that DC and macrophages display distinct patterns of cytokine regulation, particularly with respect to IL-1, as a consequence of cell-type specific differences in the physicochemical properties of the P2X(7)R PMID: 26068648
    15. Data suggest the role of stromal cell IL-1alpha and IL-1beta in Kawasaki disease vasculitis model. PMID: 26515418
    16. IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
    17. IL-1alpha-positive cells were identified in the epithelium in dextran sulfate sodium (DSS)-induced colitis. IL-1alpha was detected in the stool of colitic mice before IL-1beta. PMID: 25864926
    18. IL-1alpha acts as an alarmin essential for leukocyte recruitment and protective immunity against HSV-1 PMID: 25323745
    19. The aim of this study was to characterize the role of IL-1 in cellular responses of carbon nanotubes in cells from IL-1alpha/beta wild type (IL1-WT) mice. PMID: 25748835
    20. These findings do not support the previously suggested role of nuclear IL-1alpha in gene regulation of IL-1beta. PMID: 25748836
    21. the underlying mechanism by which AR influences AAA development is through IL-1alpha and transforming growth factor-beta1, and provides a potential new therapy to suppress/prevent AAA by targeting AR with ASC-J9. PMID: 26324502
    22. inhibition of this potentially important source of chronic inflammation in atherosclerosis requires blockade of interleukin-1alpha and not interleukin-1beta. PMID: 26139463
    23. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PMID: 25058342
    24. data suggest that central inhibition of IL-1alpha or Tox3 overexpression during the acute phase of a CNS insult may be an effective means for preventing the loss of neurological function PMID: 26224856
    25. Data (including date from studies in knockout mice) suggest that neutralization/deletion of Il1a reduces Il1b production and neutrophil infiltration in lung after inhalation exposure to silica nanoparticles. PMID: 25497724
    26. The controlled release of IL-1alpha could be a critical regulator during acute liver inflammation PMID: 25870999
    27. it appears the balance between TPO and IL-1alpha determines the MK cellular programming for thrombopoiesis in response to acute and chronic platelet needs. PMID: 25963822
    28. In acute lung injury, lipopolysaccharide induced alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1alpha release. PMID: 25862090
    29. IL-1alpha induced the proliferation of CD11b(low) alveolar macrophages and differentiated these cells into CD11b(high) macrophages which perform critical phagocytic functions and organize granuloma. PMID: 25421226
    30. Data indicate that interleukin-1 cytokines IL-1alpha and IL-1beta are regulated by polyubiquitination and proteasomal degradation. PMID: 25371210
    31. IL-1 is a key mediator driving an innate immune response to inflammatory challenge in the mouse brain but is dispensable in extracerebral tissues including the lung and peritoneum. PMID: 25367678
    32. findings identify IL-1alpha as a crucial early danger signal triggering post-MI inflammation. PMID: 25505286
    33. Immune complexes inhibit IL-1 secretion and inflammasome activation. PMID: 25320279
    34. Selective deficiency of IL-1alpha in Kupffer cells reduces liver inflammation and expression of inflammatory cytokines, which may implicate Kupffer cell-derived IL-1alpha in steatohepatitis development. PMID: 24582082
    35. cigarette smoke-induced neutrophilia was dependent on IL-1alpha produced by alveolar macrophages and alveolar macrophages isolated from smoke-exposed mice were primed for excessive IL-1alpha production in response to bacterial ligands. PMID: 25092891
    36. tested the possible role of the proinflammatory cytokine IL-1 in the age-related exhaustion of ovarian reserve using IL-1alpha and IL-1beta-KO mice PMID: 25114230
    37. This study documents distinct roles for IL-1alpha and IL-1beta in the response to Pseudomonas aeruginosa infection as a function of the type 3 secretion system effectors produced by the infecting strain. PMID: 25069982
    38. necroptosis caused the processing and release of IL-1alpha, and this was independent of IL-1beta processing and release PMID: 24790078
    39. IL-1 induces systemic inflammation and augments Streptococcal pneumoniae infection, atherosclerosis, and ischemic brain injury via platelet activation and microvascular coagulation. PMID: 24644058
    40. IL-1alpha was not released upon inflammasome activation unless significant cell damage occurred. PMID: 23684408
    41. haematopoietic-derived IL-1 is a key driver of ischaemic brain injury. PMID: 23519030
    42. In severe S. aureus bacteraemia in mice, TNF-alpha, IL-1alpha, and KC are biomarkers predicting fatal outcome of infection. PMID: 23520553
    43. Our results establish IL-1alpha as a critical initiator of the inflammatory response to L. pneumophila PMID: 23686480
    44. discovery of a novel inflammatory circuit in which RIP1-mediated IL-1alpha secretion in response to deregulated SHP-1 activity triggers an inflammatory destructive disease that proceeds independently of inflammasomes and programmed necrosis PMID: 23708968
    45. Results suggest importance of IL-1R1/IL-1alpha to the recruitment and activation of dendritic cells in response to cigarette smoke exposure. PMID: 22992200
    46. ERalpha-regulated repression of TNFalpha and IL1alpha is important for lumen formation and maintenance. PMID: 22328525
    47. Allergic sensitization to HDM was abolished in vivo when IL-1alpha, GM-CSF, or IL-33 was neutralized. PMID: 22802353
    48. Autophagy has a potentially pivotal role to play in the induction and regulation of inflammatory responses by innate immune cells, largely driven by IL-1 and its consequential effects on IL-23 secretion. PMID: 22972933
    49. Vascular wall resident cells are the main targets for the pro-atherogenic effects of bone marrow-derived IL-1 through IL-1R1, partly by induction of adhesion and chemotactic molecules in endothelial cells. PMID: 22236482
    50. This study demonstrated here in in vivo experiments that IL-1 exacerbates the effects of SCI by accentuating the impact of the inflammatory responses. PMID: 22483094

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：