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Mouse Macrophage-Derived Chemokine,MDC ELISA kit

  • 中文名称：
    小鼠巨噬细胞来源的趋化因子(MDC/CCL22)酶联免疫试剂盒
  • 货号：
    CSB-E04661m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        CCL22是一种趋化因子，也称为室内过敏原-4（MDC-4）。它主要由多种免疫细胞产生，如巨噬细胞、树突状细胞和内皮细胞等，能够通过结合CCR4受体引导CCR4受体表达的细胞向其所在的组织移动。CCL22在炎症、自身免疫和肿瘤免疫等过程中发挥着重要作用，因此被广泛研究作为治疗疾病的潜在靶点。
        j9九游会登录入口首页生物所提供的Mouse Macrophage-Derived Chemokine,MDC ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆、细胞培养上清、细胞裂解液样本中的CCL22，其灵敏度为23.672 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 别名：
    Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    23.672 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 83-92    
    1:2 Average % 100    
    Range % 92-103    
    1:4 Average % 94    
    Range % 81-98    
    1:8 Average % 99    
    Range % 90-103    
  • 回收率：
    The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 99 89-103    
    EDTA plasma (n=4) 93 84-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.888 2.812 2.850 2.683    
    1000 2.589 2.513 2.551 2.384    
    500 2.110 2.168 2.139 1.972    
    250 1.463 1.446 1.455 1.288    
    125 0.810 0.827 0.819 0.652    
    62.5 0.537 0.554 0.546 0.379    
    31.25 0.320 0.311 0.316 0.149    
    0 0.169 0.165 0.167 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
        可以简单地将CCL22描述为一种趋化因子（chemokine），属于家族中的CC型趋化因子。CCL22主要由单核细胞和树突状细胞产生，并在调节免疫细胞的迁移和定位中发挥作用。CCL22与其特定的受体CCR4结合，参与了过敏性疾病、炎症和肿瘤的发生发展过程。最新研究表明，CCL22-CCR4信号通路的抑制可能有助于治疗一些肿瘤类型。
     
  • 功能：
    Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
  • 基因功能参考文献：
    1. the subset of peritoneal CD11b(+)CD169(+) macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis PMID: 28466432
    2. Intratumoral administration of anti-CCL22 antibody inhibited B16F10 melanoma growth. PMID: 28668835
    3. this study shows that intravenous injection of apoptotic cells induces a subsequent increase in CCL22 expression and CCR4+ Treg cells, which contribute to the maintenance of immune homeostasis at least partially by splenic CD8alpha+ CD103+ dendritic cells PMID: 26868141
    4. CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
    5. CCL22 was localised mainly on the cell surface and or in the cytoplasm. Within sections of omental milky spot micrometastases, CCR4 was recognised on or in gastric cancer cells, constituent cells milky spots, blood cells and blood endothelial cells PMID: 25245466
    6. results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
    7. CCL22 has a role in Treg skin homing to suppress depigmentation PMID: 25634358
    8. CCL22 is a novel mediator of lung inflammation following hemorrhage and resuscitation PMID: 25136780
    9. The immunomodulatory properties of CCL22 could be harnessed for prevention of graft rejection and type 1 diabetes as well as other autoimmune disorders. PMID: 25740943
    10. Data indicate macrophage-derived chemokine CCL22 as an adjuvant could enhance the immune protective effect of NTHi-P6 protein vaccine to an extent. PMID: 25200152
    11. islet expression of CCL22 recruits Tregs and attenuates autoimmune destruction of beta cell PMID: 21737880
    12. Data show that the presence of the Ccr4 and Ccl22 transcripts were detected in brain slices. PMID: 21177120
    13. These results suggest that CCL22 functions to regulate development of experimental autoimmune encephalomyelitis through macrophage chemoattraction and effector function. PMID: 20940325
    14. role of CCL22 for the recruitment of eosinophils during allergic pleurisy PMID: 12629149
    15. functionally increased expression during Salmonella enterica serovar Typhimurium infection of dendritic cells PMID: 15731072
    16. Regulatory elements of the CCL22 gene required to mediate a strong and highly specific expression are included in a 4.1-kb promoter region, with the major elements active in dendritic cells and B cells being located in a small proximal 250-bp region. PMID: 15843561
    17. CCL22 was selectively upregulated in osteoclast-like cells derived from RAW264.7 cells and mouse bone marrow cells upon stimulation with RANKL (receptor activator of nuclear factor-kappaB ligand). PMID: 16821125
    18. IgE appears to be capable of stimulating basophils to produce MDC in the absence of a specific Ag, which may contribute to IgE-mediated and/or Th2-predominant allergic inflammation. PMID: 18832724
    19. When isolated, only natural killer (NK)-containing cellular fractions secrete CCL22, and the same fraction isolated from metastatic Lewis lung carcinoma (LLC)-bearing lungs secrete higher levels. PMID: 19234170
    20. Mice genetically deficient in CCR4 (the receptor for CCL22) show markedly reduced indoleamine dioxygenase (IDO) expression in MLN-DCs and support the involvement of the CCL22/CCR4 axis in IDO induction. PMID: 19843945

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.
  • 数据库链接：