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  4. Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

  • 中文名称：
    小鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒
  • 货号：
    CSB-E12639m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's mouse pulmonary surfactant-associated protein C (SP-C) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SP-C in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SP-C in samples. The color intensity is measured at 450 nm via a microplate reader.

    SP-C (SFTPC) is expressed only in the lung and is a highly specific marker for identifying TII cells. SP-C has several functions in pulmonary surfactant, including increasing the adsorption and spreading of phospholipids at the air-liquid interface thereby promoting the surface tension-lowering properties of surfactant, a role in surfactant recycling and homeostasis, and involvement in the modulation of the innate defense system. Mutations in SP-C protein and its total absence can lead to interstitial lung disease.

  • 别名：
    Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
  • 缩写：
    SFTPC
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    2.3 ng/mL-150 ng/mL
  • 灵敏度：
    0.58 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %91
    Range %85-96
    1:4Average %95
    Range %90-100
    1:8Average %98
    Range %92-102
  • 回收率：
    The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-98
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1502.147 2.052 2.100 2.009
    751.693 1.576 1.635 1.544
    37.51.121 1.044 1.083 0.992
    18.80.648 0.639 0.644 0.553
    9.40.429 0.424 0.427 0.336
    4.70.295 0.279 0.287 0.196
    2.30.194 0.179 0.187 0.096
    00.092 0.090 0.091 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 亚细胞定位：
    Secreted, extracellular space, surface film.
  • 数据库链接：