Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Tri-iodothyronine,T3 ELISA Kit

Mouse Tri-iodothyronine,T3 ELISA Kit

  • 中文名称：
    小鼠三碘甲状腺原氨酸(T3)酶联免疫试剂盒
  • 货号：
    CSB-E05086m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        T3是一种甲状腺激素，由甲状腺合成。它通过与核受体结合影响细胞代谢和生长。T3可以影响人体的许多生理过程，包括心脏功能、体温调节和代谢率。它也可以影响大脑的发育和功能。在体内，T3的浓度受到甲状腺激素合成和代谢的调节。T3的作用机制复杂，涉及多个通路和生物分子的相互作用。
        j9九游会登录入口首页生物所提供的Mouse Tri-iodothyronine,T3 ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的T3，其灵敏度为0.5 ng/mL，检测范围为0.5 ng/mL-8 ng/mL。
     
  • 缩写：
    T3
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.5 ng/mL-8 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%

     

    Three samples of known concentration were tested twenty times on one plate to assess.

     

    Inter-assay Precision (Precision between assays): CV%<15%

     

    Three samples of known concentration were tested in twenty assays to assess.

      

  • 标准曲线：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  • 本试剂盒所含材料：
      • An assay plate --- The 96-well plate has been pre-coated with an anti-mouse T3 antibody.
      • Standard (6 x 1 ml) --- Reconstitute one of the standards with sample diluent at dilution series and draw the standard curve.
      • Biotin-conjugated T3 (1 x 6 ml)
      • HRP-avidin (1 x 6 ml)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
      • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away the unbound solution and non-specific substances.
      • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Stop Solution (1 x 7 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strip (For 96 wells) --- Cover the microtiter plate when incubation.
      • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    显示更多

    收起更多

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶点详情

  • 最新研究进展：
    T3是一种重要的甲状腺激素，对于机体的发育、生长和代谢具有重要作用。最新的研究表明，T3可以通过多种信号通路调节细胞的增殖、分化和凋亡等生命活动，同时还能够影响多个重要的代谢通路。