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  4. Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

  • 中文名称：
    小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)酶联免疫试剂盒
  • 货号：
    CSB-E04739m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        TNFRSF1B，全名为肿瘤坏死因子受体家族成员 1B（TNF receptor superfamily member 1B），也称为TNFR2。TNFR2的分布比TNFR1更为局限，主要存在于免疫细胞表面，其信号通路与TNFR1类似，但与细胞增殖和凋亡的调节有关的分子和通路不同。TNFR2的调节和作用机制与TNFR1存在着复杂的相互影响和调节。
        j9九游会登录入口首页生物所提供的Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的TNFRSF1B，其灵敏度为1.6 pg/mL，检测范围为7.8125 pg/mL-500 pg/mL。
     
  • 别名：
    Tnfrsf1b; Tnfr-2; Tnfr2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD antigen CD120b
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8125 pg/mL-500 pg/mL
  • 灵敏度：
    1.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFsR-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 91    
    Range % 86-95    
    1:40 Average % 97    
    Range % 92-102    
    1:80 Average % 90    
    Range % 87-98    
    1:160 Average % 92    
    Range % 85-96    
  • 回收率：
    The recovery of mouse TNFsR-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 93 89-97    
    EDTA plasma (n=4) 98 95-101    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.858 2.911 2.885 2.777    
    250 2.360 2.250 2.305 2.197    
    125 1.485 1.455 1.470 1.362    
    62.5 0.785 0.812 0.799 0.691    
    31.25 0.482 0.521 0.502 0.394    
    15.625 0.270 0.263 0.267 0.159    
    7.8125 0.189 0.187 0.188 0.080    
    0 0.104 0.112 0.108 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    TNFRSF1B又称为肿瘤坏死因子受体2（TNF receptor 2），它可以结合肿瘤坏死因子（TNF）并转导信号，参与炎症、细胞增殖和凋亡等生物学过程。近年来的研究表明，TNFRSF1B在肿瘤的免疫治疗中起到了重要作用，研究人员正在探索利用TNFRSF1B作为免疫治疗靶点的可能性。
  • 功能：
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
  • 基因功能参考文献：
    1. MPTP induced TNF-alpha signaling through TNFR2 mediated pathway and recruited p65-p52 dimer in hippocampal nucleus which is reported to have protective effect on hippocampal neurons indicated by unchanged neuronal count in hippocampus in treated groups with respect to control. PMID: 29481885
    2. study reports an important role for TNFR2 on low-affinity-primed secondary effector CD8+ T cells; results demonstrate the importance of TNF signaling in low-affinity, cross-reactive CD8+ T cell responses during heterologous immunity PMID: 27481849
    3. our findings implicate TNFR2 in supporting myeloid-derived suppressor cells -mediated immune suppression and metastasis in the liver PMID: 26483205
    4. TNFR2 blockade appears to disrupt commensal bacteria-host immune symbiosis to reveal autoimmune demyelination in genetically susceptible mice. PMID: 26475926
    5. TNFR2 protects mice from colitis by inhibiting the expansion of colonic CD8(+) T cells. PMID: 26072395
    6. To determine the role of TNF-TNFR2/p75 signaling in ischemia-induced inflammation and muscle regeneration, we subjected wild-type and TNFR2/p75 knockouts to hind limb ischemia. PMID: 25466901
    7. Results demonstrate novel roles of TNFRII in the regulation of Abeta production, suggesting a potential therapeutic strategy for Alzheimer's disease by up-regulating TNFRII levels and elevating phosphorylated IkappaBalpha by SUMOylation. PMID: 24824215
    8. PGRN protection of endoplasmic reticulum stress induced apoptosis was abolished when TNFR2 signaling was blocked. PMID: 24703938
    9. TNFR2 signaling by itself is both sufficient and required to induce Vbeta5+ Treg expansion. PMID: 25098294
    10. TNF-alpha produced by alveolar macrophages induces Th2 cell response, via TNFR2 on Natural killer T cells. PMID: 24191851
    11. both young and aged mice deficient of TNFR2 performed poorly in the contextual fear conditioning test and displayed decreased anxiety-like behavior PMID: 24135018
    12. Mice lacking TNFRp75 exhibit greater control of M. tuberculosis infection compared with WT mice. TNFRp75-/- mice developed effective bactericidal granulomas and demonstrated increased pulmonary recruitment of activated dendritic cells. PMID: 24569452
    13. TNFR2 deficiency significantly impaired transmembrane TNF-alpha-induced release of IL-10 and NO and inhibition of T cell proliferation by MDSC supernatant. TNF-alpha acts as a potent activator of MDSCs via TNFR2 and promotes tumor immune escape. PMID: 24379122
    14. Our results indicate that TNFR2 signaling is required for the innate airway hyperresponsiveness that develops in obese mice, and suggest that TNFR2 may act by promoting IL-17A, endothelin, and/or trkB expression. PMID: 22584291
    15. In immune-mediated inflammatory bowel disease models, TNFR2 signaling increases long MLCK expression, resulting in tight junction dysregulation, barrier loss, and induction of colitis. PMID: 23619146
    16. High Soluble TNFR2 is associated with infarct size and ventricular dysfunction in ST-elevation myocardial infarction PMID: 23405158
    17. We propose a novel mechanism in which TNF activates TNFR2 on Treg cells and thereby expands this immunosuppressive immune cell population. PMID: 23385062
    18. In a chronic model of proliferative nephritis, IRF1 and renal-expressed TNFR2 were essential for sustained macrophage accumulation. PMID: 23623383
    19. Lipopolysaccharide-induced pulmonary alveolar inflammation upregulates alveolar macrophage-mediated production of sTNFR p75. Mechanical ventilator-induced lung injury enhances alveolar epithelial permeability leading to increased sTNFRp75. PMID: 21512145
    20. results suggest that TNFR2 expressed on podocytes and its canonical NF-kappaB signaling may directly interpose the compound pathogenic responses by podocytes to TNF-alpha, in the absence of other TNFR2-positive renal cell types in proliferative podocytopathies PMID: 21221075
    21. p75 is essential for tumor angiogenesis and survival in highly vascularized murine lung tumor xenografts. PMID: 22158049
    22. Both internalization and ASK1-interacting protein-1 association are required for TNFR2-dependent JNK and apoptotic signaling in endothelial cells. PMID: 22743059
    23. this suggests that the protective effect seen in mice lacking TNFR2 results rather from environment-induced attenuation by low dose bacterial endotoxins than from missing pro-inflammatory signalling via the TNFR2. PMID: 22449555
    24. TNF-alpha signaling through TNFR2 is essentially involved in the bile acid synthesis and excretion of lipids, resulting in its beneficial effects. PMID: 21850461
    25. co-activation of TNFR2 during beta-AR stress is anti-inflammatory and counterbalances pro-inflammatory, pro-hypertrophic effects of unopposed TNFR1 activation PMID: 21691899
    26. expression on non-haematopoietic cells is required for Foxp3+ Treg-cell function and disease suppression in EAE PMID: 22105853
    27. TNFR1, not TNFR2, mediates TNF-alpha stimulation of dendritic cell maturation and CD8 T cell response to recombinant mouse hepatitis virus infection in vivo. PMID: 21709152
    28. requirement for TNFR2-expression on murine natural Tregs (nTregs) and induced Tregs (iTregs) in mediating suppression of colitis PMID: 21572024
    29. These results provide the first in vivo evidence for the origin and regulation of soluble TNFR2 in serum. PMID: 21258364
    30. TR2 is an important mediator in colitis, and may serve as a therapeutic target in inflammatory bowel disease. PMID: 21347711
    31. Tumor necrosis factor-alpha signaling via TNFR1/p55 is deleterious whereas TNFR2/p75 signaling is protective in adult infarct myocardium PMID: 21153348
    32. Mesenchymal stem cell TNFR2 plays an important role in MSC-mediated cardiac protection after myocardial ischemia. PMID: 20160664
    33. critical for developing macrophages that control of T-cell proliferation PMID: 20518824
    34. These data indicate that, through TNFR2, microglia may contribute to the counter-regulatory response activated in neuropathological conditions. PMID: 20600925
    35. These data suggest a protective role for TNFR2/Fas in the pathogenesis of traumatic brain injury. PMID: 20205514
    36. Intratumoral Tregs expressing more TNFR2 are able to overcome the greater resistance to suppression of intratumoral conventional T cells, resulting in a dominant immunosuppressive tumor environment. PMID: 20525892
    37. Mouse aorta smooth muscle cells differentiate into lymphoid tissue organizer-like cells on combined tumor necrosis factor receptor-1/lymphotoxin beta-receptor NF-kappaB signaling. PMID: 20139367
    38. TNFR2(KO) myocardium showed little Etk activation and cell cycle entry but increased ASK1 activation and apoptosis. PMID: 19788501
    39. TNF type 2 receptor (p75) lowers the threshold of T cell activation PMID: 11739497
    40. Interaction of TNFR2 with TNF is important in the pathogenesis of wasting associated with acute graft-versus-host-disease (GVHD)and in the generation of histopathological lesions of intestinal GVHD. PMID: 11884480
    41. CD120b signaling is required for optimal CD40-induced IgM secretion by B cells. PMID: 11907088
    42. Review. Blockade of the p75TNFR may be helpful in treating systemic autoimmunity since its function may be essential for systemic tissue damage. PMID: 12220546
    43. Requirement for tumor necrosis factor receptor 2 expression on vascular cells to induce experimental cerebral malaria. PMID: 12228317
    44. p75 TNF-alpha receptor 2 is required for the regulation of airway hyperresponsiveness (AHR) by gamma delta T cells, since p75-deficient mice develop AHR to a similar degree as control mice following sensitization and challenge with allergen. PMID: 12370348
    45. TNFRs within the CNS play a significant role in shaping the outcome of TNF signaling during neuroimmune interactions. PMID: 12522266
    46. Role differs fom that of p55TNFR in the induction of PAI-1. PMID: 12598326
    47. LPS triggers a rapid shedding of CD120b via a protein kinase C- and Src-independent pathway mediated by p38 MAPK, furin, and metalloproteinase. PMID: 12663667
    48. TNF/TNFR2 interactions facilitate generation of FasL-dependent CTL effector pathways that play an important role in in vivo antiviral-immune responses in the liver. PMID: 12960267
    49. Skin tumour multiplicity was significantly reduced in TNFR1-/- and TNFR2-/- mice compared to wild-type mice. PMID: 14661063
    50. Impaired induction of major histocompatibility complex class I glycoproteins in TNF-R2-deficient mice following axotomy of facial motor nucleus underscores the central role of TNF in the immune activation of the injured nervous system. PMID: 14961564

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 数据库链接：