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Mouse carcinoembryonic antign,CEA ELISA Kit

  • 中文名称:
    小鼠癌胚抗原(CEA)酶联免疫试剂盒
  • 货号:
    CSB-E13925m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Mouse CEACAM5 ELISA Kit was designed for the quantitative measurement of Mouse CEACAM5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名:
    Ceacam5 ELISA Kit; Psg30Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; Pregnancy-specific glycoprotein 30 ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    62.5 pg/mL-4000 pg/mL
  • 灵敏度:
    15.6 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-93
    1:2Average %98
    Range %95-100
    1:4Average %102
    Range %97-107
    1:8Average %94
    Range %91-96
  • 回收率:
    The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-105
    EDTA plasma (n=4)9390-97
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.522 2.635 2.579 2.402
    20001.656 1.764 1.710 1.533
    10001.047 1.095 1.071 0.894
    5000.637 0.604 0.621 0.444
    2500.366 0.390 0.378 0.201
    1250.275 0.281 0.278 0.101
    62.50.212 0.230 0.221 0.044
    00.171 0.183 0.177
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
  • 基因功能参考文献:
    1. a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
  • 亚细胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
  • 蛋白家族:
    Immunoglobulin superfamily, CEA family
  • 数据库链接:

    KEGG: mmu:73250

    UniGene: Mm.244138