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  4. Mouse diamine oxidase,DAO ELISA Kit

Mouse diamine oxidase,DAO ELISA Kit

  • 中文名称：
    小鼠二胺氧化酶(DAO)酶联免疫试剂盒
  • 货号：
    CSB-E10090m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse DAO ELISA Kit was designed for the quantitative measurement of Mouse DAO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 mU/mL-1000 mU/mL and the sensitivity is 3.9 mU/mL.

  • 别名：
    Aoc1 ELISA kit; Abp1Amiloride-sensitive amine oxidase [copper-containing] ELISA kit; DAO ELISA kit; Diamine oxidase ELISA kit; EC 1.4.3.22 ELISA kit; Amiloride-binding protein 1 ELISA kit; Amine oxidase copper domain-containing protein 1 ELISA kit; Histaminase ELISA kit
  • 缩写：
    ABP1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 mU/mL-1000 mU/mL
  • 灵敏度：
    3.9 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-87
    1:2Average %95
    Range %91-99
    1:4Average %91
    Range %88-96
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 109104-112
    EDTA plasma (n=4)8581-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    10002.879 2.752 2.816 2.607
    5002.301 2.216 2.259 2.050
    2501.584 1.560 1.572 1.363
    1250.945 0.932 0.939 0.730
    62.50.655 0.672 0.664 0.455
    31.20.532 0.542 0.537 0.328
    15.60.412 0.407 0.410 0.201
    00.211 0.206 0.209
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse DAO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled DAO antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

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问答及客户评论

■ 常见问题解答
Q:

小鼠DAO ELISA试剂盒使用什么稀释血清?

A:
我们4倍稀释检测血清和血浆，这仅供参考。不同的样品采集时间不同，检测和稀释条件也会有所不同。我们建议您先做一个预测试，以确定最佳稀释倍数。

靶点详情

  • 功能：
    Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Copper/topaquinone oxidase family
  • 数据库链接：