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  4. Mouse luteinizing hormone (LH) ELISA kit

Mouse luteinizing hormone (LH) ELISA kit

  • 中文名称：
    小鼠黄体生成素（LH）酶联免疫试剂盒
  • 货号：
    CSB-E12770m
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
    The Mouse luteinizing hormone (LH) ELISA kit is designed for detection and quantitation of mouse LH in serum, plasma, or tissue homogenates. It has been validated with high sensitivity, strong specificity, good linearity, precision less than 15%, and consistency across batch. Refer to the product instructions for more details.
     
    The measurement of this assay is based on the Sandwich-ELISA tecnique. The samples and biotin-labeled LH antibody are successively pipetted into the wells pre-coated with anti-mouse LH antibody. HRP-avidin is subsequently added to the wells. After thorough washing, the substrate TMB is added for color development. TMB is converted to blue by HRP and to yellow by the stop solution. The intensity of color is positively correlated with the LH content in the samples.
     
    LH is a gonadotropin hormone released from the anterior pituitary. It stimulates the synthesis of sex steroids, which in turn negatively regulate the release of hypothalamic GnRH and further LH production. LH contributes to the maturation of primordial germ cells in both sexes. In male, LH causes the Leydig cells of the testes to produce testosterone. While LH triggers the creation of steroid hormones from the ovaries in female. LH is also involved in the modulation of the length and order of the menstrual cycle in females.
  • 别名：
    LhbLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
  • 缩写：
    LH
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
  • 检测范围：
  • 灵敏度：
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse LH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Six vials lyophilized standard (0.5 ml/bottle)--- Dilute a bottle ofthe standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled LH antibody 1 x 6 ml---Act as the detection antibody.
      • HRP-avidin 1 x 11ml --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml --- Chromogenic agent A acts as a hydrogen donor.
      • Substrate B 1 x 7 ml --- Chromogenic agent B (including TMB) is used for color developing.
      • Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我用CSB-E12770m检测小鼠血清，标曲od值为0.1-1.7，可以

By 温老师

靶点详情

  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：