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  4. Mouse pulmonary surfactant-associated protein D (SP-D) ELISA kit

Mouse pulmonary surfactant-associated protein D (SP-D) ELISA kit

  • 中文名称：
    小鼠肺表面活性蛋白D(SP-D)酶联免疫试剂盒
  • 货号：
    CSB-E12640m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; Lung surfactant protein D ELISA Kit
  • 缩写：
    SP-D
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %82-92
    1:2Average %95
    Range %90-100
    1:4Average %97
    Range %91-101
    1:8Average %93
    Range %89-99
  • 回收率：
    The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-95
    EDTA plasma (n=4)9892-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.135 2.052 2.094 1.923
    501.762 1.676 1.719 1.548
    251.317 1.234 1.276 1.105
    12.50.844 0.839 0.842 0.671
    6.250.569 0.527 0.548 0.377
    3.120.336 0.319 0.328 0.157
    1.560.211 0.207 0.209 0.038
    00.173 0.169 0.171 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please provide the following information: 1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agents such as antiseptic or preservative? If included, what is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.

CSB-E12640m
This kit is designed against full length. Pls refer to: https://www.uniprot.org/uniprot/P50404.
Standard is 293 cell-derived recombinant protein.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.Stop solution is 4N sulfuric acid.
Preservatives is liquid proclin 300(1:3000).
The kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.

Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • 基因功能参考文献：
    1. Colitis caused a notable increase in Sftpd gene expression in the gallbladder, but not in the lung, via the activity of glucocorticoids produced in the liver. PMID: 28878025
    2. SP-D deficiency reduces atherosclerosis in ApoE-knockout mice by decreasing the accumulation and proliferation of macrophages and by reducing IL-6 levels systemically. PMID: 28472244
    3. Our data may promote further studies on the morphological development of the aging lung and the role of SP-D in this process. PMID: 28185902
    4. The experimentally induced alterations were more severe in the SP-D(-/-) than in the WT mice, particularly with respect to the surfactant-storing lamellar bodies which were sometimes extremely enlarged in SP-D(-/-) mice. PMID: 27565967
    5. these results demonstrate SP-D plays protective roles by inhibiting apoptosis and modulating NF-kappaB-mediated inflammation in CLP-induced acute pancreatic injury . PMID: 26634656
    6. our results demonstrate that SFTPD deficiency affects female fertility, highlighting roles for SFTPD in ovarian and uterine physiology PMID: 26874827
    7. the collectin Surfactant Protein D is an essential component of host innate immunity to helminths PMID: 26900854
    8. SP-D protects the ocular surface from S. aureus infection. PMID: 26398197
    9. Estrogen positively regulates expression of SP-D in the mouse uterus. Progesterone, along with estrogen synergizes SP-D expression, however, when administered alone results in negative regulation. PMID: 25693975
    10. NOS2 mediates remodeling of septal walls, resulting in deposition of interstitial tissue in Sftpd(-/-). PMID: 26320150
    11. Suggest a protective role for SP-D in the pathogenesis of sub-epithelial fibrosis in a mouse model of allergic inflammation through regulation of eosinophil-derived TGF-beta1. PMID: 25472740
    12. Data indicate that although iNOS is involved in radiation-induced injury and altered SP-D structure, in the absence of SP-D, it functions to promote proinflammatory signaling. PMID: 25552309
    13. No effect of ablation of surfactant protein-D on acute cerebral infarction in mice. PMID: 25038795
    14. These results establish that DPPI is a major determinant of survival following Klebsiella pneumoniae lung infection and suggest that the survival disadvantage in DPPI(+/+) mice is in part due to processing of surfactant protein D by DPPI. PMID: 24955853
    15. Together, these studies support the conclusion that surfactant protein D increases susceptibility to Cryptococcus neoformans infection by promoting Cryptococcus neoformans-driven pulmonary IL-5 and eosinophil infiltration. PMID: 24478083
    16. Sp-D was identified as the ligand of Ig-Hepta/GPR116. PMID: 23922714
    17. Surfactant protein D contributes to ocular defense against Pseudomonas aeruginosa in a murine model of dry eye disease. PMID: 23762428
    18. Serum levels of SP-D increase during lung injury, with a sustained increment during chronic inflammation compared with acute inflammation. PMID: 23435873
    19. SP-D participates in host defense against the vaccinia virus infection by interaction with the viral surface protein A27. PMID: 23518578
    20. SP-D can protect the bladder urothelium against UPEC infection and suggest a possible function of SP-D in urinary tract. PMID: 23012359
    21. Mice lacking SP-D were partially protected during Cryptococcus neoformans infection; they displayed a longer mean time to death and decreased fungal burden at several time points postinfection than wild-type mice. PMID: 22547543
    22. The data suggest SP-D as a regulator of energy intake and body composition and an inhibitor of metabolic endotoxemia. SP-D may play a causal role at the crossroads of inflammation, obesity, and insulin resistance. PMID: 22509382
    23. Inflammation is initiated from abnormal alveolar epithelial cells in Hermansky-Pudlak syndrome, and S-nitrosylated SP-D plays significant role in amplifying pulmonary inflammation. PMID: 21616998
    24. a unique biological relevance for SP-D-DNA interactions and places SP-D as an important innate immune protein that promotes bacterial trapping by NETs during neutrophil-mediated host defense. PMID: 21724991
    25. SP-D inhibits pulmonary inflammation and migration of peripheral monocyte/macrophages into the lung through GM-CSF-dependent pathways during indirect lung injury PMID: 20639460
    26. Viruses expressing the hemagglutinin of pandemic influenza A viruses were associated with significant pathology in the lower respiratory tract, including acute inflammation, and showed low binding activity for lung surfactant protein D. PMID: 21334038
    27. SP-D decreases allergen responses, an effect that may be mediated by increase of CTLA4 in T cells. PMID: 20435925
    28. Complementation of pulmonary abnormalities in SP-D(-/-) mice with an SP-D/conglutinin fusion protein PMID: 11956209
    29. Results suggest that SP-D-dependent processes regulating surfactant lipid homeostasis were disassociated from those mediating emphysema. PMID: 12163500
    30. SP-D-deficient mice have an up to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages with excess surfactant phospholipid accumulation that is reversible by intrapulmonary administration of a fragment of recombinant SP-D. PMID: 12218102
    31. SP-D binds to apoptotic cells in a localized, patchy pattern, drives apoptotic cell ingestion by phagocytes through a mechanism dependent on calreticulin and CD91, and alters apoptotic cell clearance from the naive lung. PMID: 12244199
    32. CRP-ductin binds gram-positive and gram-negative bacteria and interacts with lung surfactant protein D. PMID: 12884308
    33. Surfactant-associated protein D plays a role in host defense against Pneumocystis carinii in vivo by modulating clearance of organisms, lung inflammation, and metabolism of NO. PMID: 15073692
    34. surfactant protein D gene transcription is regulated by NFAT and thyroid transcription factor-1 PMID: 15173172
    35. local cytokine release is regulated by SP-D when the (IAV) Influenza A virus is glycosylated at a specific site (N165) suggesting a role in protection from IAV infection. PMID: 15280465
    36. GM-CSF-dependent macrophage activity is not necessary for emphysema development in SP-D-deficient mice PMID: 15310555
    37. SP-D regulates uptake and catabolism by type II cells and influences the ultrastructure of surfactant in the alveolus. PMID: 15579631
    38. SP-D influences innate host defense, in part, by regulating sCD14 in a process mediated by MMP-9 and MMP-12. PMID: 15814723
    39. Sp-D has an important role in the regulation of cytokine milieu and eosinophilia in the lungs, and its absence highlights its essential role in host defense against pulmonary allergenic challenge. PMID: 15905537
    40. SP-D can bind free DNA and DNA present on apoptotic and necrotic cells, enhance their phagocytosis, and minimize autoantibody generation. PMID: 15905582
    41. serum SP-D can serve as a biomarker of lung inflammation in both acute and chronic lung injury in mice PMID: 15967375
    42. Our data provides evidence that SP-D has a significant role to play in the clearance of pneumococci during the early stages of infection in both pulmonary sites and blood. PMID: 16255775
    43. The ligand binding of homologous human, rat, and mouse trimeric trimeric neck plus carbohydrate recognition domain (neck+CRD) fusion proteins, each with identical N-terminal tags remote from the ligand-binding surface, was compared. PMID: 16514117
    44. allergen exposure induces elevation in SP-D protein levels in an interleukin-4/interleukin-13-dependent manner, which in turn, prevents further activation of sensitized T cells PMID: 16517724
    45. collagen domain of SP-D is required for the regulation of pulmonary macrophage activation, airspace remodeling, and surfactant lipid homeostasis PMID: 16787926
    46. Our data suggest that SP-D in BALF binds beta-glucan on Blastomyces dermatitidis, blocking alveolar macropahges access to beta-glucan, thereby inhibiting TNF-alpha production. PMID: 16861641
    47. SP-D regulates surface CD11b and CD11c levels on the alveolar macrophage by modulating receptor trafficking, providing a mechanism by which SP-D mediates phagocytic activity in the alveolar macrophage. PMID: 17056703
    48. The in vitro expression of SP-D gene in A549 cells prolonged cell survival following exposure to cigarette smoke condensate. PMID: 17298450
    49. Data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo. PMID: 17954522
    50. Important in ocular host defense. Interference with the action of or a deficiency in SPD, even transiently, could predispose the eye to Pseudomonas keratitis. PMID: 17972221

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
  • 蛋白家族：
    SFTPD family
  • 数据库链接：

    KEGG: mmu:20390

    STRING: 10090.ENSMUSP00000076383

    UniGene: Mm.1321