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Pig Alpha-fetoprotein(AFP) ELISA kit

  • 中文名称：
    猪甲胎蛋白(AFP)酶联免疫试剂盒
  • 货号：
    CSB-EL001421PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig AFP ELISA Kit was designed for the quantitative measurement of Pig AFP protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

  • 别名：
    AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %88-102
    1:2Average %92
    Range %87-96
    1:4Average %88
    Range %82-94
    1:8Average %97
    Range %92-102
  • 回收率：
    The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)9186-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.416 2.352 2.384 2.289
    1001.683 1.661 1.672 1.577
    500.945 0.904 0.925 0.830
    250.492 0.459 0.476 0.381
    12.50.302 0.287 0.295 0.200
    6.250.214 0.209 0.212 0.117
    3.120.151 0.145 0.148 0.053
    00.098 0.092 0.095 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma. Synthesized by the fetal liver and yolk sac.
  • 数据库链接：