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  4. Pig Angiopoietin 1,ANG-1 ELISA Kit

Pig Angiopoietin 1,ANG-1 ELISA Kit

  • 中文名称：
    猪血管生成素1(ANG-1)酶联免疫试剂盒
  • 货号：
    CSB-E08457p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 别名：
    ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6 ng/mL-200 ng/mL
  • 灵敏度：
    6 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-105    
    1:2 Average % 95    
    Range % 85-100    
    1:4 Average % 89    
    Range % 85-101    
    1:8 Average % 90    
    Range % 80-99    
  • 回收率：
    The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-105    
    EDTA plasma (n=4) 96 90-102    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    200 0.345 0.351 0.348      
    100 0.478 0.486 0.482      
    50 0.703 0.716 0.710      
    17 1.068 1.103 1.086      
    6 1.316 1.355 1.336      
    0 1.925 1.968 1.947      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • 基因功能参考文献：
    1. Angiopoietin 1 was predominantly present in the maturing glomeruli. PMID: 20598355
    2. The expression of ANGPT1 has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros. PMID: 20713984
    3. Attenuates H2O2-induced stress-activated protein kinase (SEK)1/JNK phosphorylation through the PI 3-kinase/Akt pathway and inhibits the apoptosis of vascular endothelial cells induced by oxidative stress. PMID: 16000309
    4. Estrogen-dependent control of Ang-1 expression plays an important role in stabilizing meningeal microvessel and maintaining healthy microvascular networks. PMID: 17496211
  • 亚细胞定位：
    Secreted.
  • 数据库链接：