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Plant hormone abscisic acid,ABA ELISA Kit

  • 中文名称：
    植物激素脱落酸(ABA)酶联免疫试剂盒
  • 货号：
    CSB-E09159Pl
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

        植物激素脱落酸（ABA）是一种重要的植物激素，它可以影响植物的生长、发育和对外界刺激的反应。ABA具有保护作用，可以帮助植物应对不利因素，如干旱、冷冻、逆境等。此外，ABA还能帮助控制植物生长方向。

        j9九游会登录入口首页生物所提供的Plant hormone abscisic acid,ABA ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测植物植物组织样本中的ABA，其灵敏度为0.04 μg/mL，检测范围为0.156 μg/mL-10 μg/mL。
  • 缩写：
    ABA
  • 种属：
    Plant
  • 样本类型：
    plant tissues
  • 检测范围：
    0.156 μg/ml-10 μg/ml.
  • 灵敏度：
    0.04 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<20%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 回收率：
    The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Xylem saps from plants (n=5) 95 89-98  
    Crude extracts (n=5) 95 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.124 0.126 0.125    
    5 0.213 0.228 0.221    
    2.5 0.357 0.361 0.359    
    1.25 0.498 0.506 0.502    
    0.625 0.686 0.701 0.694    
    0.312 1.244 1.268 1.256    
    0.156 1.510 1.534 1.522    
    0 2.241 2.297 2.269    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

When the samples are ground, does it matter how quickly they are processed – I have hundreds of samples and I think I will have to grind at least 96/per kit before processing a set. Is the time taken after grinding before processing further limited, just in case the sample changes its chemical composition?
My samples are wheat leaves grown under drought conditions. Some of them are small collections and I am concerned they may not reach 0.5 g as stated in the protocol. If say the sample is less than 05.g, e.g. 0.4g, does that mean I will need to use 3.6 ml of the extraction buffer, since 0.5 g to 4.5 ml is 1: 9 in ratio terms.

A:
Thanks for your inquiry. There is no strict requirement on the grind speed, but it would be better to grind the sample as faster as you can.
It is recommended to grind the sample well and then process further on testing soon.
As you mentioned that you need to prepare large QTY sample, here advice you to store the first prepared sample at 2-8 ℃。 Pls kindly note this just can be stored for one day.
If you do not make the experiment at the same time, really advice you to prepare the sample partially according to your test request.
You can check your experiment schedule and control the time for this.
In theory, the sample volume can be adapted. Generally it is recommended to take 0.5g to prepare the sample. If it is too less, the sample will not be detected.
And dear, if your sample is really not abundant, here are 2 advice: first is that pls try to collect more sample, second is that change the sample based on the ratio terms 1:9.
Q:

The freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried – please state for how this needs to be. In addition, how is the grinding performed? What tools are used?

A:
Thanks for your inquiry.
Yes, the freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried. You already keep them at -80 degrees Celsius, the samples are through the freeze-dried process indeed.
When do make the test, pls take 0.5g out and use the mortar usually used in lab to grind the freeze-dried samples.
And then check the detailed steps"Crude extracts " according to the manual to arrange the preliminary experiment.

靶点详情

  • 最新研究进展：
        植物激素脱落酸（Abscisic Acid，ABA）是一种重要的植物激素，具有调节植物生长发育、应对环境胁迫等多种生理作用。近年来，对ABA的研究逐渐深入，以下是最新的研究进展：
        ABA在植物抗旱适应中的作用机制得到了深入研究。研究发现，ABA能够调节植物根系的生长和分布，使根系更深入土壤中寻找水源。同时，ABA还能够促进植物蒸腾作用的减缓，从而减少水分流失。
        ABA的代谢和信号转导途径研究取得了新进展。研究人员已经发现多种ABA受体，并且探索了它们与ABA的结合机制。同时，ABA代谢途径中的关键酶类的作用机制也得到了更深入的研究。
        ABA在植物与环境互动中的作用机制也被深入探究。例如，研究发现ABA能够调节植物对盐碱胁迫的响应，同时还可以通过调节植物光合作用等途径来增加植物对环境的适应能力。
        ABA的应用研究也取得了一定进展。ABA已经被广泛用于植物生长调节剂和抗旱剂等领域，例如在提高作物耐旱性、改善果实品质等方面都有着广泛的应用前景。