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  4. Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

  • 中文名称：
    大鼠Ⅰ型胶原C端肽(CTX-Ⅰ)酶联免疫试剂盒
  • 货号：
    CSB-E12776r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        CTX- (C端肽交联端粒化Ⅰ型胶原) 是Ⅰ型胶原的降解产物，在体内反映骨代谢的情况，被广泛用于骨质疏松的诊断和疗效评估。CTX- 作为骨吸收的标志物，在骨疾病、肿瘤骨病、炎症性肠病等疾病的诊断和治疗过程中得到广泛应用。
        j9九游会登录入口首页生物所提供的Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的CTX-，其灵敏度为31.25 pg/mL，检测范围为25 pg/mL-2000 pg/mL。
     
  • 别名：
    C-telopeptide of type 1 collagen,CTX-1,_x000D_C-telopeptide of type I collagen,CTX-I
  • 缩写：
    CTX-
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-2000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 98  
    Range % 94-102  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-102  
    EDTA plasma (n=4) 86 82-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.353 0.354 0.354    
    750 0.468 0.475 0.472    
    375 0.616 0.605 0.611    
    125 1.011 0.995 1.003    
    25 1.512 1.594 1.553    
    0 2.013 2.114 2.064    
  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

A:
Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0；1:10；1:100 is advised.（you can consider use PBS as diluent solution）.
If any further questions,pls do not hesitate to tell me.
Q:

What's the antibody information of this kit?

A:
Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.
■ 客户评论
  • Sample type: Serum
    Sample species: Rat
    Review:Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. PMID: 29326134
  • Sample type: Plasma
    Sample species: Rat
    Review: Enzyme-linked immunosorbent assay (ELISA) kits were used to determine leptin and adiponectin, insulin and C-peptide , osteocalcin, ALP, CTX, and NTX, and estradiol levels in Molecules 2018, 23, 1205 11 of 14 plasma according to the manufacturer's protocol.
    PMID: 29772836
  • Sample type: Serum
    Sample species: Rat
    Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.
    PMID: 29108027
  • Sample type: Serum
    Sample species: Rat
    Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

靶点详情

  • 最新研究进展：
    CTX，全称为胶原交联肽，是一种骨代谢标记物，用于评估骨代谢和骨质疏松症等骨疾病的风险。最新的研究显示，CTX与骨转换有关，在骨吸收和骨生成的过程中扮演重要的角色。CTX的水平也与肝脏疾病、肾脏疾病、糖尿病和代谢综合症等多种疾病有关。近年来，CTX已成为评估骨质疏松症治疗效果的重要指标。