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  4. Rat Erythropoietin,EPO ELISA Kit

Rat Erythropoietin,EPO ELISA Kit

  • 中文名称：
    大鼠红细胞生成素(EPO)酶联免疫试剂盒
  • 货号：
    CSB-E07323r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat EPO ELISA Kit was designed for the quantitative measurement of Rat EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    EpoErythropoietin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %95
    Range %91-99
    1:4Average %96
    Range %89-101
    1:8Average %94
    Range %85-99
  • 回收率：
    The recovery of rat EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-100
    EDTA plasma (n=4)9586-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.176 2.191 2.184 2.075
    51.642 1.624 1.633 1.524
    2.51.236 1.218 1.227 1.118
    1.250.842 0.859 0.851 0.742
    0.6250.537 0.522 0.530 0.421
    0.3120.373 0.381 0.377 0.268
    0.1560.154 0.162 0.158 0.049
    00.108 0.110 0.109 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat EPO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled EPO antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能参考文献：
    1. Increases in plasma erythropoietin and erythropoietin receptor activation are mechanisms implicated in the increase of plasma FGF23 in acute kidney injury. PMID: 29395333
    2. Epo and Epo-Receptor are expressed in the spiral ganglion neurons (SGNs) of the inner ear of the rat, and Epo adenovirus vector (AdEpo) can decrease the spontaneous apoptosis of SGNs, which may provide a basis for the prevention or alleviation of sensorineural hearing loss. PMID: 29436578
    3. EPO administration increased TMPRSS6 content in the plasma membrane-enriched fraction of liver homogenate; in iron-pretreated rats, this increase was abolished. The results confirm that iron pretreatment prevents the EPO-induced decrease in liver Hamp expression. PMID: 28282554
    4. Protection of erythropoietin markedly attenuated pyroptosis and apoptosis of neurons exposed to sevoflurane via Erk1/2-Nrf2/Bach1 signal pathway. PMID: 28189515
    5. protective effects of EPO may be mediated by nitric oxide and the expression of different NOS enzymes, especially iNOS isoform PMID: 28087117
    6. EPO receptor ligand dimeric peptides AF41676 and AF43136 administered before reperfusion are able to reduce infarct size in a rat model of AMI. PMID: 27521836
    7. The present study aimed to determine the expression of erythropoietin (EPO) and the EPO receptor (EPOR) in spiral ganglion neurons (SGNs) in the inner ear of rats of various ages, and the associated neuronal apoptosis and hearing alterations. PMID: 27959434
    8. Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
    9. Taken together, our results reveal that EPO and cEPO mediate MEF2 target gene expression via the regulation of HDAC5 phosphorylation at Ser259/498, and suggest that HDAC5 could be a mechanism contributing to the therapeutic actions of EPO and cEPO. PMID: 26777998
    10. this study is the first to elucidate the detailed mechanism by which AhR plays an indispensable role in the suppression of HIF activation by IS. PMID: 26561638
    11. the hypercapnic hypoxia causes maximum activation of the synthesis and accumulation of erythropoietin HIF-1alpha, and permissive hypercapnia enhances their content more than hypoxic exposure. PMID: 26852593
    12. Results of the present study demonstrate the neuroprotective, anti-inflammatory and antioxidant effects of EPO in a rotenone-induced neurodegenerative animal model. PMID: 25464888
    13. Vitamin A deficiency leads to ineffective erythropoiesis by the down-regulation of renal erythropoietin expression in the kidney, resulting in erythrocyte malformation and the consequent accumulation of the heme group in the spleen. PMID: 24998947
    14. EPO partially rescued insulin-stimulated AKT activation, reduced oxidative stress, and restored heat-shock protein 72 expression in soleus muscles. PMID: 25767056
    15. Results showed that the inhibitory effects of TNFa on the hypoxia-induced upregulation of erythropoietin expression are mediated primarily by HIF-2a rather than HIF-1a PMID: 25283246
    16. Data suggest a potential positive effect of erythropoietin (EPO) on hepatocytes (HCs) proliferation during liver regeneration after a partial hepatectomy. PMID: 24928528
    17. HIF-1alpha/EPO hypoxic signal transduction may initiate hippocampal neurogenesis following hypoxia-ischemia PMID: 25935977
    18. The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074
    19. Epidermal growth factor and erythropoietin infusion accelerate functional recovery in combination with rehabilitation. PMID: 24763928
    20. In IN-IC cells, hypoxia increased mRNA expression of erythropoietin, erythropoietin concentration in the medium and protein expression of HIF PHD2. PMID: 24832733
    21. After 2-weeks of hypoxia, erythropoietin transcripts and erythropoietin plasma levels were significantly increased in hypoxic animals compared with control animals. PMID: 24524370
    22. rMSCs transplantation could be a new therapy for degenerative retinal diseases since it can protect and rescue RPE and retinal neurons, while EPO gene modification to rMSCs could be an even better option. PMID: 23971737
    23. Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis. PMID: 23728385
    24. EPO induces the expression of myelin genes in oligodendrocytes and this effect requires the presence of EPOR. PMID: 23821361
    25. EPO significantly suppresses the enhanced apoptosis and activity of caspase 3 that are induced by 6-hydroxydopamine. PMID: 22294054
    26. 11 weeks of endurance training increased erythrocyte deformability. Epo and EpoR may contribute to the decreased morphological index and deformability indicator in erythrocytes during endurance training in rats. PMID: 22495316
    27. EPO potently protects against cardiopulmonary bypass-induced acute renal injury by inhibiting expression of NF-kappaB p65 and inflammatory response. PMID: 22659586
    28. EPO induces a significant decrement in the ohmic airway resistance. PMID: 22766248
    29. Pyruvate activation of the hypoxia-inducible factor-1alpha-EPO signaling cascade in neurons and glia could protect the brain from ischemia-reperfusion injury PMID: 22282883
    30. Ligustilide exerts neuroprotective effects against I/R injury by promoting EPO transcription via an ERK signalling pathway and inhibiting RTP801 expression. PMID: 21410687
    31. A protective cascade for erythropoietin (EPO) relies upon modulation of Wnt1, Akt1, FoxO3a, GSK-3beta, beta-catenin, and mitochondrial apoptotic pathways for the development of new strategies against DM vascular complications. PMID: 21443457
    32. Our work identifies novel pathways for EPO in the vascular system that can govern the activity of SIRT1 PMID: 21722091
    33. EPO, hCG, or their combination ameliorate cardiac remodeling post-myocardial infarct in a rat model PMID: 21707946
    34. Epo combined with granulocyte colonystimulating factor enhanced Mmp2 expression in mesenchymal stem cells, promoted motility and activated the ERK1/2 signaling pathway. PMID: 21461559
    35. EPO mediates brain-derived neurotrophic factor-dependent sonic hedgehog (SHH) expression and 3-nitropropionic acid (3-NP) resistance because suppression of EPO activity by soluble EPO-receptor abolishes BDNF-mediated SHH induction and 3-NP resistance. PMID: 20580927
    36. The expression of erythropoietin in the hippocampus increased from 3 to 18 months and decreased from 18 to 30 months. PMID: 19779256
    37. EPO may play a role in normal spinal cord development by regulating cell proliferation and apoptosis. PMID: 20167254
    38. Changes in EPO concentration [EPO] was evaluated every hour after single exposure of 10% O(2) for 120 min in order to determine a peak level of [EPO]. PMID: 20204781
    39. Intermittent hypoxic exposure can enhance serum hypoxia inducible factor-1 alpha and erythropointin levels. PMID: 20104680
    40. Our results support a model in which Epo promotes Schwann cell migration and assembly of the provisional extracellular matrix in the injured peripheral nerve by its effects on integrin recruitment to the cell surface and local FN production. PMID: 19705458
    41. All regions of kidney express erythropoietin receptors. Review. PMID: 12381912
    42. role in preventing the induction of Apaf-1 and preserving mitochondrial membrane potential in conjunction with enhanced Bcl-XL expression. EPO also inhibits the activation of caspase-9. PMID: 12621307
    43. EPO inhibits apoptosis in ventricular myocytes exposed to hypoxia, through an Akt-dependent pathway PMID: 12927817
    44. Erythropoietin may provide benefit after hypoxic-ischemic events in the developing brain, a major contributor to static encephalopathy and cerebral palsy. PMID: 13129593
    45. Hypoxic acclimatization can inhibit the increment of erythropoietin gene expression induced by acute hypoxia in rat hepatic and renal tissues. PMID: 14706194
    46. EPO mediates the classic neurotrophic effects of proliferation, differentiation and maintenance in a dopaminergic cell line. PMID: 15050726
    47. Treatment of neural progenitor cells with erythropoietin stimulated progenitor cell differentiation into neurons, but not astrocytes. PMID: 15167538
    48. These data demonstrate that EPO can promote differentiation of neuronal stem cells into astrocytes in an EPO receptor dependent manner, and this effect may be associated with the activation of ERK kinase and NF-kappaB pathway. PMID: 15249201
    49. Age-related decreases in Erythropoietin may contribute to degenerative events following age-related decreases in brain flow and oxygen supply. PMID: 15262216
    50. Study demonstrates how in response to axonal injury, rat periaxonal Schwann cells release erythropoietin (EPO), which via EPO receptor binding on neurons, prevents axonal degeneration. PMID: 15470751

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 组织特异性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 数据库链接：