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  4. Rat High density lipoprotein,HDL ELISA Kit

Rat High density lipoprotein,HDL ELISA Kit

  • 中文名称：
    大鼠高密度脂蛋白(HDL)酶联免疫试剂盒
  • 货号：
    CSB-E11703r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat HDL ELISA Kit was designed for the quantitative measurement of Rat HDL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

  • 别名：
    N/A
  • 缩写：
    HDL
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    78 ng/mL-5000 ng/mL
  • 灵敏度：
    19.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 99  
    Range % 96-102  
    1:400 Average % 103  
    Range % 100-107  
    1:800 Average % 89  
    Range % 84-96  
    1:1600 Average % 96  
    Range % 94-97  
  • 回收率：
    The recovery of rat HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-99  
    EDTA plasma (n=4) 103 99-107  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    5000 2.511 2.620 2.566 2.444  
    2500 1.863 1.870 1.867 1.745  
    1250 1.344 1.236 1.290 1.168  
    625 0.756 0.787 0.772 0.650  
    312.5 0.521 0.560 0.541 0.419  
    156 0.358 0.356 0.357 0.235  
    78 0.226 0.230 0.228 0.106  
    0 0.118 0.125 0.122    
  • 数据处理：
  • 货期：
    3-5 working days

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