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  4. Rat Immunoglobulin G,IgG ELISA Kit

Rat Immunoglobulin G,IgG ELISA Kit

  • 中文名称：
    大鼠免疫球蛋白G(IgG)酶联免疫试剂盒
  • 货号：
    CSB-E07981r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

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  • 产品描述：
        IgG是一种免疫球蛋白，广泛存在于人体的血浆和组织液中，占所有免疫球蛋白中数量最多的一种。它有很多生物学功能，比如保护机体免受感染、清除异物和毒素、识别和结合病原体等。此外，IgG还参与许多免疫反应的调节，比如激活补体、介导细胞介导的毒性和抗体依赖性细胞介导的细胞毒性等。因此，IgG的检测对于疾病的诊断和治疗具有重要意义。
        j9九游会登录入口首页生物所提供的Rat Immunoglobulin G,IgG ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆样本中的IgG，其灵敏度为31.25 ng/mL，检测范围为31.25 ng/mL-8000 ng/mL。
     
  • 别名：
    N/A
  • 缩写：
    IgG
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    31.25 ng/mL-8000 ng/mL
  • 灵敏度：
    31.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 100  
    Range % 95-104  
    1:2000 Average % 90  
    Range % 86-95  
    1:4000 Average % 87  
    Range % 83-92  
    1:8000 Average % 101  
    Range % 97-106  
  • 回收率：
    The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-95  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.230 0.225 0.228    
    2000 0.417 0.407 0.412    
    500 0.643 0.664 0.654    
    125 1.238 1.257 1.248    
    31.25 1.754 1.770 1.762    
    0 2.035 1.988 2.012    
  • 本试剂盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG.
      • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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靶点详情

  • 最新研究进展：
    IgG是一种免疫球蛋白，是人体免疫系统中最常见的抗体类型。它可以识别和结合到外来物质，如病毒、细菌和其他病原体，从而帮助身体清除这些病原体。最近的研究表明，IgG不仅可以发挥免疫作用，还可以在其他方面发挥作用，如参与血小板功能调节、影响血脑屏障通透性、调节神经元发育等。此外，人体在某些疾病或免疫系统失调状态下，会产生抗IgG抗体，这些抗体会与IgG结合并影响其功能，相关研究也得到了不断的深入。