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  4. Rat TNF-α ELISA kit
  • 中文名称：
    大鼠肿瘤坏死因子α（TNF-α）酶联免疫试剂盒
  • 货号：
    CSB-E11987r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        TNF（肿瘤坏死因子）是一种细胞因子，由多种免疫细胞产生，包括巨噬细胞、T细胞和B细胞。TNF参与了机体的免疫调节、炎症反应等多种生理和病理过程，与多种疾病的发生和发展有关，如肿瘤、自身免疫性疾病、感染性疾病等。TNF也是一种炎症标志物，可以用于评估机体的炎症反应情况。
        j9九游会登录入口首页生物所提供的Rat TNF-α ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的TNF，其灵敏度为1.56 pg/mL，检测范围为6.25 pg/mL-400 pg/mL。
     
  • 别名：
    Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 87-96  
    1:2 Average % 99  
    Range % 95-104  
    1:4 Average % 101  
    Range % 97-105  
    1:8 Average % 98  
    Range % 93-107  
  • 回收率：
    The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 80-92  
    EDTA plasma (n=4) 99 95-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.037 2.067 2.052 1.871  
    200 1.445 1.489 1.467 1.286  
    100 1.017 1.034 1.026 0.845  
    50 0.655 0.677 0.666 0.485  
    25 0.411 0.438 0.425 0.244  
    12.5 0.351 0.378 0.365 0.184  
    6.25 0.297 0.282 0.290 0.109  
    0 0.183 0.179 0.181 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：大鼠

稀释比：没有稀释

产品评价： 因为要补数据，时间又比较紧急，就找武汉j9九游会登录入口首页生物做代测服务。寄样到武汉很方便，出结果也很快。标曲拟合很完美，样本结果整体可以，能够满足补充需求，听说他们的实验工程师、技术支持都是经验丰富的免疫学相关技术人员，结果比较可靠。推荐~~~

By 陈老师

问答及客户评论

■ 常见问题解答
Q:

我想知道正常大鼠的正常血清TNFa浓度，以及实验大鼠诱导游离佐剂RA的血清TNFa浓度，以ng/ml为单位。另外我还想问一下，实验动物在诱导弗氏佐剂关节炎RA期间7周内的体重损失，以g为单位。

A:
我们使用CSB-E11987r大鼠TNF-α ELISA试剂盒来测量正常大鼠血清/血浆样本。具有严重炎症的模型甚至可以达到数百pg/ml的水平。结果会因样品不同条件和不同生物模型而异。至于您上述提到的诱导模型，我们对样品条件不太确定。具体以客户实测为准。 至于您关于体重损失的问题，通常取决于您自己的实验目的或目标的选择。如果您只想测试TNF-α，那么仅使用TNF-α elisa kit检测就可以了。
■ 客户评论
  • Sample type: PVN tissue
    Sample species: Rat
    Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.
    PMID: 30978539
  • Sample type: Myocardial cells
    Sample species: Rat
    Review: TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni's post hoc test. n = 3 independent experiments.
    PMID: 30953402
  • Sample type: Serum and lung tissue
    Sample species:Rat
    Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.
    PMID: 30362554
  • Sample type: Serum
    Sample species: Rat
    Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile,serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation [Color figure can be viewed at wileyonlinelibrary.com].
    PMID: 31020644
  • Sample type: Colorectal tissues
    Sample species: Rat
    Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.
    PMID: 30319255
  • Sample type: Liver homogenate
    Sample species: Rat
    Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumour necrosis factor- , interlukin-1 and inducible nitric oxide synthase.
    PMID: 30823534
  • Sample type: Serum
    Sample species: Rat
    Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions.
    PMID: 30466624
  • Sample type: Cell culture supernates
    Sample species: Rat
    Review:The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.
    PMID: 30675709
  • Sample type: Spinal cord tissue
    Sample species: Rat
    Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.
    PMID: 31006904
  • Sample type: Bronchoalveolar lavage fluid
    Sample species: Rat
    Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.
    PMID: 30892077
  • Tested application: ELISA
    Test sample: Serum,Tissue homogenate: heart tissue homogenate
    Species: Rat
    Review: The standard was worked perfectly in my experiment but I could don get much difference in control and disease group animal serum samples.
  • Sample type: Serum
    Sample species: Rat
    Review: The results were remarkable and the repeatability of the kit was phenomenal.

靶点详情

  • 最新研究进展：
    TNF（肿瘤坏死因子）是一种重要的细胞因子，在炎症反应、免疫调节、细胞凋亡等方面具有重要作用。最近的研究表明，TNF参与了多种疾病的发生发展，如类风湿关节炎、炎症性肠病、心血管疾病等。研究还表明，TNF对肿瘤的抑制和促进作用也是不可忽视的。
  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：