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  4. Rat Testosterone,T ELISA Kit

Rat Testosterone,T ELISA Kit

  • 中文名称：
    大鼠睾酮(T)酶联免疫试剂盒
  • 货号：
    CSB-E05100r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Testosterone (T) ELISA Kit was designed for the quantitative measurement of Rat Testosterone (T) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.13 ng/mL-25.6 ng/mL and the sensitivity is 0.06 ng/mL.

  • 别名：
    N/A
  • 缩写：
    T
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.13 ng/mL-25.6 ng/mL
  • 灵敏度：
    0.06 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-104  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 89  
    Range % 83-93  
  • 回收率：
    The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-95  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    25.6 0.238 0.236 0.237    
    6.4 0.435 0.431 0.433    
    2 0.611 0.615 0.613    
    0.5 0.984 0.982 0.983    
    0.13 1.403 1.398 1.401    
    0 2.001 2.003 2.002    
  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    T4，全称为甲状腺素（thyroxine），是由甲状腺合成和分泌的一种甲状腺激素。T4在人体内具有重要的生理作用，它能够促进蛋白质、脂肪和碳水化合物的代谢，并参与调节心血管、神经、消化、泌尿系统等多种生理功能。近年来，T4的研究热点主要集中在其与疾病的关联性，如甲状腺功能减退症、自身免疫性甲状腺疾病、心血管疾病等。此外，T4也被广泛应用于临床上，如用于甲状腺激素替代治疗和辅助诊断甲状腺相关疾病等。