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Rat Thyroid Stimulating Hormone,TSH ELISA Kit

  • 中文名称：
    大鼠促甲状腺素(TSH)酶联免疫试剂盒
  • 货号：
    CSB-E05115r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
        TSH (促甲状腺激素，thyroid-stimulating hormone) 是垂体腺细胞产生的一种促进甲状腺合成和释放甲状腺素的激素。在人体内，TSH的含量是由甲状腺激素水平负反馈控制的。当甲状腺激素水平下降时，下丘脑和垂体腺释放TRH和TSH来促进甲状腺合成和释放甲状腺素。
        j9九游会登录入口首页生物所提供的Rat Thyroid Stimulating Hormone,TSH ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的TSH，其灵敏度为0.3 μIU/mL，检测范围为0.6 μIU/mL-24 μIU/mL。
     
  • 别名：
    TshbThyrotropin subunit beta ELISA kit; Thyroid-stimulating hormone subunit beta ELISA kit; TSH-B ELISA kit; TSH-beta ELISA kit; Thyrotropin beta chain ELISA kit
  • 缩写：
    TSH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.6 μIU/mL-24 μIU/mL
  • 灵敏度：
    0.3 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 87-98  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 91  
    Range % 85-96  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of rat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-100  
    EDTA plasma (n=4) 93 88-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    24 2.756 2.781 2.769    
    12 1.997 1.988 1.993    
    6 1.142 1.124 1.133    
    3 0.634 0.613 0.624    
    1.2 0.244 0.253 0.249    
    0.6 0.115 0.117 0.116    
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat TSH antibody.
      • Standard 7 x 0.5 ml--- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated TSH antibody 1 x 6 ml --- Bind to the TSH in the sample or in the standard and react with the TMB substrate to make the solution chromogenic.
      • Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
      • Substrate A1 x 7 ml --- Mix with Substrate B, and the mixture reacts with HRP to make the solution appear blue.
      • Substrate B 1 x 7 ml --- Mix with Substrate A, and the mixture reacts with HRP to make the solution appear blue.
      • Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：大鼠

稀释比：其他 1：10

产品评价： 我用CSB-E05115r检测自身免疫性甲状腺炎大鼠，稀释比例为1：10。甲功的elisa盒子操作步骤一样，同一批血清可以同时做，大大缩短实验时间，非常方便，结果准确。

By 丁老师

问答及客户评论

■ 常见问题解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶点详情

  • 最新研究进展：
    TSH是一种由垂体前叶分泌的激素，它通过刺激甲状腺合成和释放甲状腺激素来调节代谢。最新的研究表明，TSH不仅影响代谢，还可能在其他生理和病理过程中发挥重要作用。例如，TSH受体在某些肿瘤中表达增加，TSH可能通过调节肿瘤细胞增殖和生存来影响这些肿瘤的发展。此外，还有研究表明TSH可能与心血管疾病、精神疾病和骨代谢等方面有关。
  • 功能：
    Indispensable for the control of thyroid structure and metabolism.
  • 基因功能参考文献：
    1. These data add new insight to an important role of T3 in rapidly regulating TSH beta gene expression at posttranscriptional level. PMID: 21078364
    2. TSH action stimulates the phosphorylation of hsp-90 in FRTL-5 thyroid cells PMID: 16910874
    3. Results clearly show that there are diurnal changes in TSH expression and accumulation in rat PT-TSH cells and suggest that these fluctuations are regulated by melatonin. PMID: 17927662
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：

    KEGG: rno:25653

    STRING: 10116.ENSRNOP00000022575

    UniGene: Rn.9966