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  4. Rat Tumor necrosis factor β,TNF-β ELISA KIT

Rat Tumor necrosis factor β,TNF-β ELISA KIT

  • 中文名称：
    大鼠肿瘤坏死因子β(TNF-β)酶联免疫试剂盒
  • 货号：
    CSB-E07379r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TNF-β ELISA Kit was designed for the quantitative measurement of Rat TNF-β protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 别名：
    Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
  • 缩写：
    TNF-β
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 96-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 87
    Range % 83-94
    1:8 Average % 96
    Range % 91-102
  • 回收率：
    The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 99108
    EDTA plasma (n=4) 88 84-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.632 2.765 2.699 2.614
    250 2.212 2.302 2.257 2.172
    125 1.545 1.577 1.561 1.476
    62.5 0.765 0.771 0.768 0.683
    31.2 0.411 0.423 0.417 0.332
    15.6 0.257 0.264 0.261 0.176
    7.8 0.176 0.185 0.181 0.096
    0 0.084 0.085 0.085 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 基因功能参考文献：
    1. Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780
    2. TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID: 12849708
  • 亚细胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：