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  4. Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

  • 中文名称：
    大鼠血管内皮细胞生长因子(VEGF)酶联免疫试剂盒
  • 货号：
    CSB-E04757r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        VEGFA（血管内皮生长因子A，vascular endothelial growth factor A）是血管生成和血管修复过程中的重要因子，能促进血管内皮细胞增殖和血管生成。
        j9九游会登录入口首页生物所提供的Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的VEGFA，其灵敏度为0.97 pg/mL，检测范围为3.9 pg/mL-250 pg/mL。
     
  • 别名：
    Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 pg/mL-250 pg/mL
  • 灵敏度：
    0.97 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 84-101  
    1:2 Average % 94  
    Range % 90-100  
    1:4 Average % 96  
    Range % 91-103  
    1:8 Average % 94  
    Range % 86-100  
  • 回收率：
    The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-97  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    250 2.712 2.892 2.802 2.614  
    125 2.369 2.317 2.343 2.155  
    62.5 1.568 1.604 1.586 1.398  
    31.25 0.877 0.888 0.883 0.695  
    15.6 0.558 0.562 0.560 0.372  
    7.8 0.371 0.384 0.378 0.190  
    3.9 0.276 0.286 0.281 0.093  
    0 0.188 0.187 0.188 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat VEGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled VEGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    VEGFA是一种血管生成因子，可以促进血管内皮细胞增殖和血管形成。VEGFA在许多生理和病理过程中都发挥重要作用，如胚胎发育、血管生成、肿瘤生长和转移等。近年来，研究人员发现VEGFA还与多种疾病如心血管疾病、眼科疾病、糖尿病等密切相关。
  • 功能：
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
  • 基因功能参考文献：
    1. Our main findings demonstrate that 2-h swim exercise rapidly (i.e., immediately postexercise) increased Vegf-a mRNA levels in both subcutaneous (iWAT) and intra-abdominal (eWAT, rpWAT) adipose tissue of male Wistar rats after 2 weeks of high fat diet but not in rats kept on low fat diet. PMID: 29486133
    2. the epigenetic mechanisms involved in the physical exercise-mediated induction of VegfA expression, were examined. PMID: 27975171
    3. Our data suggest that activation of the GPR91-ERK1/2-C/EBP beta (c-Fos, HIF-1a) signaling pathway plays a tonic role in regulating VEGF transcription in rat retinal ganglion cells. PMID: 28374767
    4. present results suggested that pathologically high cyclic stretch induces the shuttling of SRSF1 which results in the secretive pattern splicing of VEGFA and finally contributes to the proliferation of vascular smooth muscle cells. PMID: 29974845
    5. Results indicate that vascular endothelial growth factor (VEGF) is a vital important factor that contributes to the small-airway remodelling in a rat model of Chronic obstructive pulmonary disease through promoting angiogenesis, which mainly depend on the specific binding between VEGF and VEGF receptor 1 and can be effectively attenuated by sunitinib. PMID: 28117425
    6. 150-kDa oxygen-regulated protein (ORP150) levels were significantly increased in rat retinas after induction of diabetes. Vascular endothelial growth factor (VEGF) and the pro-inflammatory cytokines interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) induced upregulation of ORP150 in HRMEC. PMID: 29098793
    7. Expression of vascular endothelial growth factor in cardiac repair: Signaling mechanisms mediating vascular protective effects PMID: 29462681
    8. VEGF-A expression in soft tissues repaired by shockwave therapy PMID: 29921384
    9. While the mRNA expression of the proangiogenic factors VEGF, transforming growth factor-beta1 (TGF-beta1), and adrenomedullin dropped at 0 h post-hypoxia compared to normoxic control, no changes were detected at the protein level, showing an impaired response of these proangiogenic factors to hypoxia in the aged striatum PMID: 28501895
    10. Kisspeptin-10 prevents the increased vascular permeability of ovarian hyperstimulation syndrome by the activation of KISS1R and the inhibition of VEGF. PMID: 28676533
    11. The results indicate that VEGF promotes endothelial progenitor cell differentiation and vascular repair through Cx43. PMID: 29065929
    12. The results obtained in the study revealed that down-regulation of VEGF by RNAi could be a novel target approach for the treatment of failure (UFF) in rats with peritoneal dialysis. PMID: 28733472
    13. VEGF165 induces differentiation of hair follicle stem cells into endothelial cells and plays a role in in vivo angiogenesis. PMID: 28244687
    14. Enhanced neuroprotective efficacy of bone marrow mesenchymal stem cells co-overexpressing BDNF and VEGF in a rat model of cardiac arrest-induced global cerebral ischemia PMID: 28492549
    15. this is the first report describing the use of novel polymeric gene carriers for the delivery of VEGF gene to DA neurons with improved transfection efficiency. Finally, the study will lead to a significant advancement in the field of non-viral Parkinson's Disease gene therapy treatment. PMID: 28025049
    16. the results indicate that, Osx-overexpression promotes ADSCs' osteogenesis both in vitro and in vivo, which could be enhanced by release of VEGF. PMID: 28107808
    17. Following IL-1beta (50mug/kg ip.) administration, a higher serum IL-1beta level was detected in females than in males. In male rats, IL-1beta decreased BDNF mRNA in all the brain structures, except for the pituitary, and VEGF mRNA in the amygdala. PMID: 27744091
    18. VEGF knockdown can enhance chondrogenesis and prevent osteoarthritis progression. PMID: 27164955
    19. Here we report for the first time the expression of a rare and unusually potent splice variant, VEGF 111 , in vivo in mammals. Our discovery of VEGF 111 in the uterus of both a eutherian (rat) and a marsupial (fat-tailed dunnart) suggests that the splice variant may be common to all mammals PMID: 27722773
    20. Data indicate vascular endothelial growth factor A (VEGFA) as a target gene of miR-200b. PMID: 28122882
    21. Our results suggest that hUCMSCs can promote ovarian expression of HGF, VEGF, and IGF-1 through secreting those cytokines, resulting in improving ovarian reserve function and withstanding ovarian senescence. PMID: 28279229
    22. We demonstrate that a fine balance of VEGF and angiopoietin signaling is required for the formation of a functional vascular network. PMID: 27868196
    23. results indicate that the paracrine factor, VEGF, derived from transplanted BM-MSCs, regulated the expression of miRNAs such as miRNA-23a and miRNA-92a and exerted anti-apoptotic effects in cardiomyocytes after MI PMID: 28662151
    24. VEGF expression increased markedly in the cerebellum and mildly in the occipital lobe following up to 4 weeks of exposure to 20-ppm methylmercury. VEGF expression was detected mainly in astrocytes of the blood brain barrier. Intravenous administration of anti-VEGF neutralizing antibody mildly reduced the rate of hind-limb crossing signs observed in MeHg-exposed rats. PMID: 28118383
    25. Overexpressed long non-coding RNA (lncRNA) ANRIL upregulates vascular endothelial growth factor (VEGF) and promotes angiogenesis by activating NF-kappa B (NF-kappaB) signaling pathway in diabetes mellitus (DM) combined with cerebral infarction (DM + CI) rats. PMID: 28060742
    26. Vitexicarpine increased rats' survival time and decreased serum alpha-fetoprotein as well as it ameliorated fibrosis and massive hepatic tissue breakdown. It attenuated hepatocellular carcinoma-induced protein and gene expression of vascular endothelial growth factor PMID: 28651490
    27. Knockdown of hepatic VEGF decreases hepatic sdf1 expression and plasma sdf1 levels. PMID: 26939868
    28. XBP1 regulates VEGF-mediated cardiac angiogenesis. PMID: 27133203
    29. a novel rodent model to reproduce much of the known phenotype of CTEPH, including the pivotal pathophysiological role of impaired vascular endothelial growth factor-dependent vascular remodeling, is reported. PMID: 28183533
    30. VEGF treatment increases expression of HGF in vitro and in vivo and that, based on a series of integrative studies, we suggest that the effects of VEGF are mediated through downstream HGF signaling. PMID: 27036872
    31. MiR-21 exerts protective effects on endothelial injury through the PTEN/VEGF pathway after acute myocardial infarction. PMID: 27708252
    32. Chemerin and VEGF play important roles in diabetic nephropathy. PMID: 27612613
    33. Vegf expression is increased in brain from ischemic rats following treadmill exercise. PMID: 27747480
    34. these results suggested that miRNA-29a suppresses cardiac fibrosis and fibroblasts proliferation via targeting VEGF-A/MAPK signal pathway implicating that miRNA-29a might play a role in the treatment of cardiac fibrosis. PMID: 27060017
    35. Data suggest that a vascular endothelial growth factor (VEGF) signaling defect possibly plays an important role in defective embryonic airway branching. PMID: 27372649
    36. the inhibitory effect of miR1405p on angiogenesis was attenuated by the overexpression of VEGFA. PMID: 27035554
    37. we aimed to investigate the effect on angiogenesis of endoglin and VEGF expression in 1, 2-dimethylhydrazine dihydrochloride (DMH)-induced rat colon carcinogenesis and evaluated the implementation of COX2 inhibitors for molecular targets on tumor considering the above-mentioned mechanisms. PMID: 27466499
    38. This study demonstrated that vegf increases in male rat with aneurysmal subarachnoid hemorrhage. PMID: 26923576
    39. This study demonstrates that hyperthyroidism may have an effect on the development of rat adrenal glands mediated by VEGF expression, angiogenesis, and apoptosis. PMID: 26708182
    40. This PHD-2-mediated transcriptional activation may be important for regulating VEGF expression through HIF-1a signaling in LCs during corpus luteum development in mammals. PMID: 26681021
    41. Results indicate that chronic stress enhances vulnerability of brain-derived neurotrophic factor and vascular endothelial growth factor response to neuroinflammation. PMID: 26396035
    42. Identify a mechanism of VEGF overexpression in liver and mesentery that promotes pathologic, but not physiologic, angiogenesis, via sequential and nonredundant functions of CPEB1 and CPEB4. PMID: 26627607
    43. The increased level of VEGF expression in the nicotine-treated group may have affected endothelial cell apoptosis PMID: 27363062
    44. TP53, BCL-2, and VEGFA genes may participate in the proliferation of esophagus cancer cells in a synergistic manner PMID: 26439224
    45. results indicate OVX may induce 3-D capillary regression in soleus muscle through an imbalance between VEGF-A and TSP-1 expression, possibly associated with decreased exercise tolerance in ovariectomized rats. PMID: 26092525
    46. PHD2-mediated transcriptional activation may be one of the mechanisms regulating VEGF expression in luteal cells during mammalian corpus luteum development. PMID: 25975603
    47. Cardiomyocyte VEGF activates EC Notch signaling, facilitating GPIHBP1-mediated translocation of LPL across EC and regulating LPL-derived fatty acid delivery to the cardiomyocytes. PMID: 26586663
    48. Expression in control and blank groups was similar at 3 and 6 months. Atorvastatin can inhibit VEGI and vascular endothelial growth factor expression to protect rats from diabetic retinopathy PMID: 26125742
    49. VEGF is implicated in the pathogenesis of inflammation after electrical burns. PMID: 25466960
    50. No significant differences were noted in the expression of VEGF in rat tympanic membrane perforation. PMID: 25920966

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 组织特异性：
    Expressed in the pituitary, in brain, in particularly in supraoptic and paraventricular nuclei and the choroid plexus. Also found abundantly in the corpus luteum of the ovary and in kidney glomeruli. Expressed in the ductal epithelial cells of post-pubert
  • 数据库链接：

    KEGG: rno:83785

    STRING: 10116.ENSRNOP00000026637

    UniGene: Rn.1923