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  4. Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

  • 中文名称：
    大鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)酶联免疫试剂盒
  • 货号：
    CSB-E07299r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Keratinocyte Autocrine Factor/Amphiregulin (KAF/AR) ELISA Kit is designed to measure Amphiregulin (AR) in rat serum, plasma, and tissue homogenates with exceptional accuracy and sensitivity.

    With a detection range of 1.25 pg/mL-80 pg/mL and a sensitivity of 0.31 pg/mL, this quantitative assay can detect even the smallest amount of KAF/AR in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

    This kit is ideal for researchers investigating the role of KAF/AR in cancer、activates intracellular signaling, lung morphogenesis, breast bone tissue and oocyte maturation, and tissue integrity recovery after infection or injury（激活细胞内信号、肺形态发生及乳腺骨组织和卵母细胞的发育成熟、感染或损伤后的组织完整性恢复）. The sandwich measurement principle allows for accurate quantification of KAF/AR in your samples, making it a valuable tool for any cancer research project. With its quick assay time, low sample volume requirement, and precise results, this kit is a must-have for any researcher studying cancer.

  • 别名：
    Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 pg/mL-80 pg/mL
  • 灵敏度：
    0.31 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-98
    1:2Average %95
    Range %91-99
    1:4Average %86
    Range %80-92
    1:8Average %96
    Range %92-102
  • 回收率：
    The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10498-108
    EDTA plasma (n=4)9792-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    802.538 2.616 2.577 2.413
    401.939 2.059 1.999 1.835
    201.364 1.386 1.375 1.211
    100.882 0.870 0.876 0.712
    50.549 0.541 0.545 0.381
    2.50.341 0.356 0.349 0.185
    1.250.253 0.258 0.256 0.092
    00.168 0.160 0.164 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
  • 基因功能参考文献：
    1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
    2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
    3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
    4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
    5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996

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  • 亚细胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Amphiregulin family
  • 数据库链接：