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  4. Rat α-Smooth muscle actin,α-SMA ELISA Kit

Rat α-Smooth muscle actin,α-SMA ELISA Kit

  • 中文名称：
    大鼠α平滑肌肌动蛋白(α-SMA)酶联免疫试剂盒
  • 货号：
    CSB-E14027r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat α-SMA ELISA Kit is engineered for accurate measurement of rat α-SMA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the α-SMA content in the sample. The color intensity is positively correlated with α-SMA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    α-SMA, also called ACTA2, is the actin isoform that predominates within vascular smooth muscle cells. The expression of α-SMA defines the phenotypic transition to myofibroblasts and is a major contributor to increased contractile force generation by these cells. α-SMA contributes to cell-generated mechanical tension and is normally restricted to cells of vascular smooth muscle. It is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are important effector cells of tissue fibrogenesis. α-SMA is important dor fibrogenesis. Additionally, α-SMA can enhance the contractile activity of myofibroblasts.

  • 别名：
    Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %90
    Range %85-94
    1:4Average %87
    Range %82-91
    1:8Average %102
    Range %97-109
  • 回收率：
    The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)9389-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.595 2.451 2.523 2.431
    251.689 1.601 1.645 1.553
    12.50.972 0.893 0.933 0.841
    6.250.493 0.512 0.503 0.411
    3.130.330 0.306 0.318 0.226
    1.560.206 0.210 0.208 0.116
    0.780.126 0.121 0.124 0.032
    00.094 0.089 0.092 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 基因功能参考文献：
    1. considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance. PMID: 27021728
    2. Data suggest that the expression of actin alpha 2 smooth muscle (alpha-SMA) plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli. PMID: 27021914
    3. Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120
    4. Smooth muscle alpha-actin is present in the cervix of virgin, 11 day, and 20 day pregnant rats. PMID: 25108144
    5. Data suggest that the myosin-II mediated drebrin A-actin exodus might be an initial event in chemical long-term potentiation (LTP) induction, triggering actin polymerization and spine enlargement. PMID: 24465547
    6. Acta2 is important for myofibroblast cells motility, contraction and regulation of signaling processes during wound healing. PMID: 24204762
    7. Data from several animal models suggest that up-regulation of expression of Tgfb1 (transforming growth factor beta 1) and Acta2 in proximal tubular cells is key to development of renal tubular fibrosis in chronic kidney disease/renal hypertension. PMID: 24601883
    8. Smooth muscle actin expression increased during lung injury induced by hyperoxia, and could play an important role in pulmonary fibrosis remodeling. PMID: 23040780
    9. Smooth muscle alpha-actin was increased on day 9 of bleomycin induced pulmonary fibrosis in rats, and is involved in the mechanism of N-acetylcysteine alleviation of idiopathic pulmonary fibrosis. PMID: 23006535
    10. novel populations of alphaSMA+ precursors of pericytes are born on the subventricular zones then migrate into the peri-infarct region PMID: 22805872
    11. Tendon cells are capable of actively contracting lax tendons through an alpha smooth muscle actin-based mechanism. PMID: 22517354
    12. Hepatocyte growth factor can relieve chronic renal failure, and this protection is associated with the down-regulation of alpha-SMA expression in mesangial cells and renal interstitium. PMID: 22680062
    13. Leptin potentiates acetaldehyde-induced hepatic stellate cell activation and transdifferentiation as evidenced by increased alpha-smooth muscle actin expression. PMID: 21294755
    14. The results suggest that alpha-SMA containing stress fibers contribute to myofibroblast arrhythmogeneicity. After ablation of this cytoskeletal component, cells lose their arrhythmic effects on cardiomyocytes. PMID: 21921266
    15. alpha-SMA expression in renal interstitial fibroblasts, i.e., myofibroblast transdifferentiation, accelerates the contraction of the tubulointerstitial fibrosis tissue via the Ca(2)+-dependent pathway PMID: 21630215
    16. Reactivation of fetal structural genes of vascular smooth muscle alpha-actin and its ectopic expression is associated with the cardiac allograft vasculopathy and fibrosis. PMID: 19411749
    17. The expression of alpha -SMA is increased dramatically during the fibrosis, and is positively associated with the expression of alpha -AR, beta(1)-AR and beta(2)-AR. PMID: 19785950
    18. Transcriptional regulation of a contractile gene by mechanical forces applied through integrins in osteoblasts PMID: 11953441
    19. smooth muscle alpha-actin expression requires a transforming growth factor-beta control element PMID: 12970361
    20. Tacrolimua and sirolimus both increased kidney transplantation TGF-beta1 and alpha-SMA levels. PMID: 15316360
    21. There was a significant increase in smooth muscle alpha-actin located around regenerating hepatic nodules. PMID: 15814362
    22. results provide the first in vivo evidence for the importance of the intronic CArG and transforming growth factor-beta1 control element cis-elements in the regulation of alpha-SMA expression in myofibroblasts in granulation tissue PMID: 15855636
    23. Myometrial expression of alpha-SM-actin mRNA and protein was high throughout pregnancy. PMID: 15972885
    24. Results identify alpha-smooth muscle actin as a mechanosensitive protein that is recruited to stress fibers under high tension. PMID: 16401722
    25. Force-mediated actin filament assembly promotes nuclear translocation of MRTF and subsequent activation of the SMA promoter to enhance SMA expression. PMID: 17456553
    26. Olfactory ensheathing cells synthesize smooth muscle alpha-actin (and a variety of actin-binding proteins including caldesmon) providing compelling evidence that these cells are different from Schwann cells. PMID: 17492622
    27. These findings indicate the existence of alpha-smooth muscle actin-positive endothelial cells in adult aortic endothelium and the possible association with progression of atherosclerosis. PMID: 19285011

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Actin family
  • 数据库链接：

    KEGG: rno:81633

    UniGene: Rn.195319