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  4. rat follicle-stimulating hormone,FSH ELISA Kit

rat follicle-stimulating hormone,FSH ELISA Kit

  • 中文名称：
    大鼠促卵泡素(FSH)酶联免疫试剂盒
  • 货号：
    CSB-E06869r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
        FSH (follicle-stimulating hormone)，是一种由垂体腺分泌的促性腺激素，对于卵巢和睾丸中的生殖细胞的发育和成熟起到重要作用。在女性，FSH促进卵泡的成熟并在排卵期间释放卵子。在男性，FSH促进睾丸中的精子的发育和成熟。生物学含义：FSH的水平可以用于评估性腺的功能和卵巢或睾丸的疾病诊断，如不育症、性腺功能低下等。
        j9九游会登录入口首页生物所提供的rat follicle-stimulating hormone,FSH ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的FSH，其灵敏度为0.039 ng/ml，检测范围为0.156 ng/ml - 10ng/ml。
     
  • 别名：
    FshbFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
  • 缩写：
    FSH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
  • 灵敏度：
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit FSH antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-rat FSH Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated FSH 1 x 6 ml -- Bind to the FSH antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

样品稀释液和洗液的作用相同吗？对于FSH ELISA试剂盒，怎么稀释血清？

A:
样本稀释液和洗液具有不同的功能，并且它们的成分也不同。 如果你的大鼠血清样品浓度较高，可以考虑使用样品稀释液。但是你应该通过进行预实验来确定最佳的稀释倍数。 --建议核对说明书是否有样本稀释液，有，则上述不改。

靶点详情

  • 最新研究进展：
    FSH是垂体前叶分泌的调节生殖系统功能的激素，其在卵巢和睾丸中的作用已被广泛研究。近期研究发现，FSH的受体在非生殖系统中也存在，并与多种疾病的发病机制相关，如肝纤维化、心血管疾病等。
  • 功能：
    Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
  • 基因功能参考文献：
    1. Study in primary rat pituitary cells demonstrates that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3' UTR. PMID: 29534107
    2. our results indicate that miR-21-3p and miR-433 can down-regulate the expression of FSHb by directly targeting the FSHb 3'UTR in rat primary pituitary cells. PMID: 28402262
    3. the upstream region of Fshb is susceptible to unsaturated long-chain fatty acids PMID: 26853521
    4. GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway. PMID: 25635942
    5. FSH acutely regulated CCND2 expression through both PKA and PI3K signaling pathways during granulosa cell proliferation and also accelerated its ubiquitination-proteasomal degradation. PMID: 23349233
    6. Data suggest that FSH (Cga/FSHb) induces clock system in granulosa cells by up-regulation of connexin 43 expression; this suggests that gap junctions are implicated in circadian clock in peripheral oscillators besides central pacemaker. PMID: 23299500
    7. Both the hormonal milieu of perimenopause and the association of dynamic perimenopausal changes in FSH and inhibin A with decreased bone mineral density have been reproduced in an animal model. PMID: 22249524
    8. FSH autoantibody could cause the reduction of inhibin B, thereby inducing hypospermatogenesis via augment of spermatogenic cell apoptosis PMID: 22564408
    9. Novel findings supporting the role of FSH in primordial follicle survival through an action on apoptosis. PMID: 22106407
    10. These results show that the synthesis of FSH isoforms possessing alpha2,6-linked sialic acid is hormonally regulated in male rats. PMID: 19464343
    11. Data suggest that FSH beta subunit expression (and LH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
    12. A potential new regulatory mechanism of the FSH signalling pathway that could occur during maturation of testicular cells. PMID: 20201104
    13. Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter. PMID: 12145338
    14. The regulation of FSHbeta is dependent on activin-mediated signaling factors in concert with pituitary-derived nuclear regulatory proteins. PMID: 12554780
    15. PACAP contributes to the selective regulation of FSHbeta expression during maturation in the male PMID: 12646491
    16. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription. PMID: 14512439
    17. FSH enhances HIF-1 activity downstream of the PI 3-kinase/AKT/Rheb/mTOR pathway in GCs and HIF-1 activity is necessary for FSH to induce multiple follicular differentiation markers PMID: 14982927
    18. SMAD3 plays an important role in the synergistic effects of activin and gonadotropin-releasing hormone and demonstrate that this synergy is mediated by a palindromic cis-element located at -266/-259 of the rFSHbeta gene promoter PMID: 15375186
    19. Smad3 is not only sufficent but also necessary for activin-mediated regulation of FSHbeta promoter. PMID: 15761025
    20. oxidative stress induces apoptosis in preovulatory follicles and the antiapoptotic effect of FSH is mediated in part by stimulation of follicular GSH synthesis and suppression of ROS production PMID: 16339198
    21. Testosterone regulates FSHbeta transcription, in part, through modulation of various components of the activin-signaling system. PMID: 17405825
    22. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. PMID: 17674129
    23. Smad3 and Pitx2 cooperate in stimulation of FSHbeta gene transcription. PMID: 18022758
    24. PI3-kinase may be involved in the negative regulation of alpha- and FSHbeta-subunit gene expression as well as cell proliferation. PMID: 18206295
    25. Dexamethasone (4 nM) for 3 weeks partially rescued LH beta/follicle-stimulating hormone beta cells. PMID: 18363807

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：