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  4. CR2 Monoclonal Antibody

CR2 Monoclonal Antibody

  • 货号：
    CSB-MA916751
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA916751 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The production of CR2 monoclonal antibody involves immunizing mice with the synthesized peptide derived from human CD21 protein. The hybridoma cells produced by fusing mouse B cells with myeloma cells are screened for CR2 antibody production. The CR2 monoclonal antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen. It is suitable for recognizing human CR2 protein in ELISA and IHC applications.

    CR2, also known as CD21, is a membrane-bound protein that is primarily expressed on the surface of B-cells. Its main function is to bind to and process complement-coated antigens, facilitating their presentation to T-cells and thus initiating an immune response. Additionally, CD21 also serves as a receptor for the Epstein-Barr virus, playing a role in viral entry and infection.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) CR2 Monoclonal antibody
  • Uniprot No.：
    P20023
  • 基因名：
  • 别名：
    C3DR antibody; CD 21 antibody; CD21 antibody; Complement C3d receptor 2 antibody; Complement C3d receptor antibody; Complement component (3d/Epstein Barr virus) receptor 2 antibody; Complement receptor type 2 antibody; CR antibody; Cr2 antibody; CR2_HUMAN antibody; CVID7 antibody; EBV receptor antibody; EBV-R antibody; Epstein Barr virus receptor antibody; Epstein-Barr virus receptor antibody; EVBR antibody; SLEB9 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human CD21
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1, Kappa
  • 纯化方式：
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU. Participates in B lymphocytes activation.; (Microbial infection) Acts as a receptor for Epstein-Barr virus.
  • 基因功能参考文献：
    1. This study shown the C3d+ microglial clusters were found in chronic but not acute multiple sclerosis. PMID: 27778395
    2. This study uncovers the origin of the effect of ionic strength on C3d-CR2 interaction and deepens the understanding of the molecular mechanism of their interaction, which is valuable for the design of vaccines and small molecule inhibitors. PMID: 27154286
    3. Binding of PF4/heparin complexes to B cells is mediated through the interaction between complement and complement receptor 2. PMID: 27412887
    4. high pERK expression and accelerated apoptosis revert within 4 weeks after beginning therapy, whereas clonal B cells unresponsive to TLR9 stimulation persist for at least 24 weeks, although they may partially rescue normal CD21 expression. PMID: 28507081
    5. Cerebrospinal fluid (CSF) soluble complement receptor 2 (sCR2) levels correlated significantly both with CSF complements C3 and C1q as well as to a disease severity measure. PMID: 27085202
    6. CR2 contributes to human Osteonecrosis of the femoral head susceptibility in Korean systemic lupus erythematosus patients. PMID: 27446959
    7. In this study, we have shown that the CR2 +24T>C polymorphism is not a risk marker for nasopharyngeal carcinoma development in the North region of Portugal. PMID: 26748973
    8. In mature B cells, CD21 transcription proceeds from a focused transcriptional start site regulated by a non-consensus TATA box, an initiator element and a downstream promoter element. PMID: 25640655
    9. Reduced expression of CD21 on CLL B-cells appears functionally relevant and was associated with poor clinical outcomes. PMID: 26452134
    10. These data suggest that rs1876453 in CR2 has long-range effects on gene regulation that decrease susceptibility to systemic lupus erythematosus. PMID: 25180293
    11. repression of the CR2/CD21 promoter can occur through one of the E-box motifs via recruitment of RP58. PMID: 25817480
    12. found that the protective CR2 genotype is correlated with lower levels CR2 mRNA as well as differences in the ratio of the long and short CR2 isoforms PMID: 25569262
    13. The paradoxical enhancement of immune responses by C3d in the absence of CD21 is due to internalization and processing of C3d into peptides that activate autoreactive CD4(+) T-helper cells. PMID: 25385064
    14. Higher C3d/C3 ratios were found for current smoker, higher age, age-related macular degeneration phenotype, and the two SNPs in the C3 gene, rs6795735 and rs2230199. PMID: 24675670
    15. s show that expression of the Epstein-Barr virus B-cell attachment receptor, CD21, in B cells that lack this receptor results in significant changes in gene expression, both before and rapidly following EBV-CD21 interaction. PMID: 24600013
    16. Our findings implicate CR2 as a susceptibility gene for nasopharyngeal carcinoma and suggest that enhanced CR2 expression may be involved in the oncogenesis and development of nasopharyngeal carcinoma. PMID: 23612877
    17. To eradicate this virus, targeting CD21 on these EBV-infected B cells is hypothesized here. PMID: 23384704
    18. Patients with primary Sjogren's Syndrome who display high frequencies of autoreactive and unresponsive CD21-/low B cells are susceptible to developing lymphoproliferation. PMID: 23279883
    19. plasma sCD21 levels are higher, while B-cell surface CD21 expression levels are lower in B-cell chronic lymphocytic leukemia (B-CLL) patients, but not in multiple myeloma (MM) patients. PMID: 22899340
    20. Transgenic mice inoculated with elk brain homogenate expressing high levels of prion (PrPC) protein die of chronic wasting disease. PMID: 23002439
    21. All subtypes of juvenile arthritis have reduced circulating levels of sCD21 and sCD23. PMID: 21328056
    22. These results suggest that CR2 plays a role in the recognition of foreign DNA during host-immune responses PMID: 22885687
    23. the 5' UTR is a novel regulatory element for the CR2 gene in which variation may alter gene function and modify the development of Systemic lupus erythematosus PMID: 22673213
    24. Prevalence of pulmonary fibrosis in patients with diffuse cutaneous systemic sclerosis inversely correlates with soluble cluster of differentiation 21 (sCD21) levels. PMID: 21052675
    25. Marginal zone B cells activated by hepatitis C virus undergo functional exhaustion associated with BCR signaling defects and overexpression of a key antiproliferative gene, and may subsequently become terminally spent CD21(low) B cells. PMID: 22678901
    26. CD21 expression is altered in multiple sclerosis (MS) patients as in other autoimmune diseases, although no evidence is found for a specific role of the CD21 gene in MS. PMID: 22137275
    27. Patients with hepatitis C virus infection who display high frequencies of unresponsive CD21(-/low) marginal zone B cells are more susceptible to developing autoimmunity and/or lymphoproliferation. PMID: 22084433
    28. CD23 oligomers appear more important in enhancing IgE synthesis and monomers lacking the tail residues fail to bind CD21 yet bind membrane IgE and down-regulate IgE synthesis. PMID: 21889131
    29. study reports a cocrystal structure of a CR2(SCR1-2):C3d complex at 3.2 angstrom resolution in which the interaction interfaces differ markedly from the previously published structure and are consistent with the biochemical data PMID: 21527715
    30. The impact of hCR2 expression on mouse B cell development and the baseline immunological status of the mice, was evaluated. PMID: 21269698
    31. Binding between Epstein Barr virus-transformed B cell line-derived exosomes and viral structural protein gp350 is dependent on CD21 expressed on the B cells. PMID: 21106852
    32. Molecular modeling approaches used to investigate disparities between the biochemical data and the X-ray structure of the CR2-C3d cocrystal result in highest-scoring solutions in which CR2 SCR1-2 is docked within the concave surface of C3d. PMID: 20951140
    33. Biophysical investigations of complement receptor 2 (CD21 and CR2)-ligand interactions reveal amino acid contacts unique to each receptor-ligand pair PMID: 20558730
    34. analysis of the physicochemical properties underlying C3d-CR2 association PMID: 20091675
    35. Staphylococcus aureus binder of Ig (Sbi) and complement receptor 2 (CR2) share overlapping binding sites on complement 3d (C3d), thus allowing the bacterial evasion molecule to block CR2 binding, thereby inhibiting the host's adaptive immune response. PMID: 20083651
    36. CR2-induced complement activation results in membrane depolarization, as indicated by annexin V binding, with kinetics similar to those of C3-fragment deposition and different from those of membrane attack complex formation. PMID: 19740327
    37. promoter activity is critically dependent on a cell type-specific repressor PMID: 11739509
    38. Redox control of EBV infection: prevention by thiol-dependent modulation of functional CD21/EBV receptor expression. PMID: 11813981
    39. crystal structure of human CD21; implications for epstein-barr virus and c3d binding PMID: 12122212
    40. The percentage of T cells expressing CD21 from all gestations was significantly higher than the adult and the percentage positive decreased with increasing gestational age. PMID: 12149502
    41. NF-kappaB signaling pathway enhances the expression of the CR2 gene, as a result of NF-kappaB proteins binding to two CR2 promoter elements. PMID: 12444129
    42. CR2 is shed mainly as a soluble CR2 complex, in which the CR2 units link covalently and react with nucleophilic agents PMID: 12713795
    43. Complement receptor 2-mediated targeting of complement inhibitors to sites of complement activation. PMID: 12813023
    44. Coligation of CD21 with the B cell antigen receptor lowers the antigen concentration threshold for eliciting protection from Fas apoptosis by 2-3 orders of magnitude. PMID: 14607925
    45. electrostatic calculations provide global and site-specific explanations of the physical causes that underlie the ionic strength dependence of C3d-CR2 association PMID: 15187133
    46. The activation of MAPKs is independently mediated by CR2 and that anti-CR2 McAb, PD98059, and Wortmanin block the activation of MAPKs, respectively. PMID: 15603708
    47. characterized the interaction between the first two short consensus repeats (SCR1-2) of complement receptor type 2 (CR2, CD21) and C3d PMID: 15713467
    48. the open V-shaped structures formed by CR2 SCR 1-2, both when free and when bound to C3d, are optimal for the formation of a tight two-domain interaction with its ligand C3d PMID: 15713468
    49. CD21 and CD23a are common targets for B lymphotropic gammaherpesviruses. PMID: 15795251
    50. Unresponsiveness to T-dependent antigen (sheep red blood cells) displayed by transgenic CR2-positive B cells is linked to increase in the stimulus level required to propel the B cell into a fully activated state, a normal humoral immune response. PMID: 15905540

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  • 相关疾病：
    Systemic lupus erythematosus 9 (SLEB9); Immunodeficiency, common variable, 7 (CVID7)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Receptors of complement activation (RCA) family
  • 组织特异性：
    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
  • 数据库链接：

    HGNC: 2336

    OMIM: 120650

    KEGG: hsa:1380

    UniGene: Hs.445757